Department of Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5;203:450-454. doi: 10.1016/j.saa.2018.06.014. Epub 2018 Jun 4.
Copper is an essential element in some biological processes in organisms, so it was important to find ways to identify trace amounts of it. Minimal amounts of copper ions can be determined in aqueous solutions in the spectrophotometric method suggested in this study. The method depends on the reaction between Cu(II) and Amaranth dye at the pH 6.0. The gradual disappearance of Amaranth colour at 520 nm occurring with an increase of Cu(II) concentration from 0.13 up to 2.0 μg cm. The molar absorptivity coefficient and Sandell's sensitivity of the complex are found to be 0.94 × 10 L mol cm, 6.8 ng cm respectively. The advantages of this method are simple, selective, and highly sensitive. The method was used for determination of copper ions in aqueous solutions containing several metal ions, where excellent agreements between reported and obtained results were achieved in aqueous solutions containing copper metal ions only. The postulated method is in an excellent agreement with the determination of Cu(II) ion concentrations by atomic absorption spectroscopy (AAS). The interference was studied to determine the copper metal ions concentration, and do not interfere with eleven of other metal ions.
铜是生物体某些生物过程中的必需元素，因此找到识别痕量铜的方法非常重要。本研究提出的分光光度法可用于在水溶液中测定极少量的铜离子。该方法基于 Cu(II)与苋菜红染料在 pH 6.0 下的反应。随着 Cu(II)浓度从 0.13 增加到 2.0 μg cm，在 520 nm 处发生苋菜红颜色逐渐消失。该络合物的摩尔吸光系数和桑德尔灵敏度分别为 0.94×10 L mol cm 和 6.8 ng cm。该方法的优点是简单、选择性好、灵敏度高。该方法用于测定含有多种金属离子的水溶液中的铜离子，仅在含有铜金属离子的水溶液中，报告值和获得值之间具有极好的一致性。提出的方法与原子吸收光谱法（AAS）测定 Cu(II)离子浓度具有极好的一致性。对干扰进行了研究，以确定铜金属离子的浓度，且 11 种其他金属离子无干扰。
