母亲的miRNA - 146a G/C rs2910164变异、艾滋病毒/艾滋病和氮氧化物污染暴露共同影响胎儿生长。

Maternal miRNA-146a G/C rs2910164 variation, HIV/AIDS and nitrogen oxide pollution exposure collectively affects foetal growth.

作者信息

Naidoo P, Naidoo R N, Ramkaran P, Muttoo S, Asharam K, Chuturgoon A A

机构信息

1 Discipline of Medical Biochemistry and Chemical Pathology, University of KwaZulu-Natal, Durban, South Africa.

2 Discipline of Occupational and Environmental Health, University of KwaZulu-Natal, Durban, South Africa.

出版信息

Hum Exp Toxicol. 2019 Jan;38(1):82-94. doi: 10.1177/0960327118781902. Epub 2018 Jun 13.

DOI:10.1177/0960327118781902

PMID:29896975

Abstract

OBJECTIVE

: Nitrogen oxide (NOx) pollution and human immunodeficiency virus (HIV)/AIDS intensify inflammation during pregnancy and linked with adverse birth outcomes (ABOs). MicroRNA (miRNA)-146a plays a crucial role in regulating inflammation in the NF-κB pathway. The G/C rs2910164 dampens miRNA-146a activity and linked with inflammatory diseases. The present study investigated whether HIV/AIDS and NOx exposure throughout pregnancy further intensifies ABO in Black South African women genotyped for the rs2910164.

METHODS

: Pregnant women ( n = 300) were subdivided into low, medium and high NOx exposure groups, genotyped for the miRNA-146a G/C rs2910164 using polymerase chain reaction-restriction fragment length polymorphism, and further stratified based on HIV status.

RESULTS

: Unstratified data (HIV+ and HIV- mothers combined): Mothers from the high NOx group with the variant C-allele had low blood iron levels ( p = 0.0238), and had babies with reduced birthweights ( p = 0.0283). As NOx increased, the prevalence of preterm birth and low birth weight also increased in mothers with the variant C-allele versus wildtype G-allele. HIV-infected mothers: In all NOx exposure groups, mothers with the variant C-allele had higher systolic blood pressure (low: p = 0.0386, medium: p = 0.0367 and high: p = 0.0109) and had babies with lower Appearance, Pulse, Grimace, Activity and Respiration scores at 1 min (low: p = 0.0190, medium: p = 0.0301 and high: p = 0.0361).

CONCLUSION

: Maternal rs2910164 variant C-allele, NOx pollution and HIV/AIDS might collectively play a role in intensifying gestational hypertension and ABO.

摘要

目的

氮氧化物（NOx）污染和人类免疫缺陷病毒（HIV）/艾滋病会加剧孕期炎症，并与不良出生结局（ABO）相关。微小RNA（miRNA）-146a在调节核因子κB（NF-κB）途径中的炎症反应方面起着关键作用。G/C rs2910164会降低miRNA-146a的活性，并与炎症性疾病相关。本研究调查了在整个孕期感染HIV/艾滋病以及接触NOx是否会在对rs2910164进行基因分型的南非黑人女性中进一步加剧ABO。

方法

将300名孕妇分为低、中、高NOx暴露组，采用聚合酶链反应-限制性片段长度多态性方法对miRNA-146a G/C rs2910164进行基因分型，并根据HIV感染状况进一步分层。

结果

未分层数据（HIV阳性和HIV阴性母亲合并）：高NOx组携带C等位基因变体的母亲血铁水平较低（p = 0.0238），其婴儿出生体重较低（p = 0.0283）。随着NOx水平升高，携带C等位基因变体的母亲与野生型G等位基因母亲相比，早产和低出生体重的发生率也增加。HIV感染母亲：在所有NOx暴露组中，携带C等位基因变体的母亲收缩压较高（低暴露组：p = 0.0386，中暴露组：p = 0.0367，高暴露组：p = 0.0109），其婴儿在1分钟时的外貌、脉搏、 grimace、活动和呼吸评分较低（低暴露组：p = 0.0190，中暴露组：p = 0.0301，高暴露组：p = 0.0361）。