Wu Si-qi, Yang Hua, Li Ping
Yao Xue Xue Bao. 2016 Jul;51(7):1060-7.
Affinity ultrafiltration coupled with liquid chromatography-mass spectrometry (LC-MS) technology is a rapid, simple and effective method for discovering active components, which is especially applicable to the complex extracts of traditional Chinese medicines (TCMs). Affinity ultrafiltration can facilitate the rapid separation of small-molecule ligands from the target macromolecules, and LC-MS contributes to the structural identification of potential active compounds. Combination of the two techniques is vital to reveal the effective materials of TCMs and helpful for the TCM-based drug discovery. In this review, according to the studies in recent years, we make a brief introduction about the application of the ultrafiltration coupled with LC-MS technology in screening active components of TCMs, including the principle, characteristics and the research advancement.
亲和超滤结合液相色谱-质谱（LC-MS）技术是一种快速、简便且有效的发现活性成分的方法，尤其适用于中药复杂提取物。亲和超滤有助于从小分子配体与目标大分子中快速分离，而LC-MS有助于对潜在活性化合物进行结构鉴定。这两种技术的结合对于揭示中药有效物质至关重要，有助于基于中药的药物发现。在本综述中，根据近年来的研究，我们简要介绍超滤结合LC-MS技术在筛选中药活性成分中的应用，包括原理、特点及研究进展。
