Bunn S J, Hanley M R, Wilkin G P
Neurosci Lett. 1985 Apr 19;55(3):317-23. doi: 10.1016/0304-3940(85)90455-0.
Specific binding of the opioid ligand [3H]etorphine was localized in cryostat sections of the rat pituitary. The binding was concentrated over the pars nervosa. Competition with partially selective unlabelled displacers indicated that the greater part of this binding was of the kappa-subtype, sensitive to inhibition by dynorphin and bremazocine. The kappa nature of the binding was confirmed using the prototype kappa ligand [3H]ethylketocyclazocine in the presence of excess unlabelled mu and delta opioid receptor ligands. Sections of the pituitary stalk caused a loss of the hypothalamic projection to the pars nervosa and a marked gliosis. Concurrent with these changes there was a significant increase in the density of kappa opioid binding. These observations suggest that the majority of opioid receptors in the pars nervosa are of the kappa subtype and are located on the pituicytes.
阿片样物质配体[3H]埃托啡的特异性结合定位于大鼠垂体的冷冻切片中。这种结合集中在神经垂体。与部分选择性未标记置换剂的竞争表明，这种结合的大部分是κ亚型，对强啡肽和布马佐辛的抑制敏感。在存在过量未标记的μ和δ阿片受体配体的情况下，使用原型κ配体[3H]乙基酮环唑新证实了结合的κ性质。垂体柄切片导致下丘脑向神经垂体的投射丧失和明显的胶质细胞增生。与这些变化同时发生的是κ阿片结合密度显著增加。这些观察结果表明，神经垂体中的大多数阿片受体是κ亚型，位于垂体细胞上。
