糖库：聚糖分析保留特性数据库。

GlycoStore: a database of retention properties for glycan analysis.

机构信息

Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore.

Institute for Glycomics, Griffith University, Gold Coast, Australia.

出版信息

Bioinformatics. 2018 Sep 15;34(18):3231-3232. doi: 10.1093/bioinformatics/bty319.

DOI:10.1093/bioinformatics/bty319

PMID:29897488

Abstract

SUMMARY

GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics. The database is built on publicly available experimental datasets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT). It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd. GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 8500 retention positions determined by: (i) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; (ii) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and (iii) capillary electrophoresis with laser induced fluorescence detection (CE-LIF). GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource. GlycoStore aims to support detailed glycan analysis by providing a resource that underpins current workflows. It will be regularly updated by expert annotation of published data and data obtained from the project partners.

AVAILABILITY AND IMPLEMENTATION

http://www.glycostore.org.

SUPPLEMENTARY INFORMATION

Supplementary data are available at Bioinformatics online.

摘要

GlycoStore 是一个经过精心整理的色谱、电泳和质谱组合数据库，包含与一系列糖蛋白、糖脂和生物治疗药物相关的 N-、O-、糖鞘脂（GSL）聚糖和游离寡糖。该数据库构建于牛津糖生物学研究所（Oxford Glycobiology Institute）和国家生物加工研究与培训研究所（National Institute for Bioprocessing Research and Training，NIBRT）开发的 GlycoBase 中公开的实验数据集之上。现在，它已经扩展到包括 A*STAR 生物加工技术研究所（Bioprocessing Technology Institute，BTI）、麦考瑞大学（Macquarie University）和 Ludger Ltd. 的最近发表的和内部数据集合。GlycoStore 提供了大约 850 个独特聚糖结构条目，这些条目由超过 8500 个保留位置支持，这些保留位置是通过以下方法确定的：（i）亲水相互作用色谱（HILIC）超高效液相色谱（U/HPLC）和反相（RP）-U/HPLC 与荧光检测；（ii）与电喷雾质谱联用（ESI-MS/MS）检测相结合的多孔石墨化碳（PGC）色谱；（iii）激光诱导荧光检测的毛细管电泳（CE-LIF）。GlycoStore 增强了 GlycoBase 中以前可用的许多功能，同时解决了该受欢迎资源的数据集合和模型的局限性。GlycoStore 旨在通过提供支持当前工作流程的资源来支持详细的聚糖分析。它将通过对已发表数据和项目合作伙伴提供的数据进行专家注释进行定期更新。