Liu Shu, Chen Rong-xin, Li Jun, Liu Xiao-man, Huang Hong-bing, Fu Qian, Wang Chang-xi, Huang Min, Li Jia-li
Yao Xue Xue Bao. 2016 Aug;51(8):1240-4.
The study aims to investigate the associations of SLCO1B1 polymorphisms with tacrolimus concentrations in Chinese renal transplant recipients. Blood samples and clinical data were collected from 89 renal transplant recipients with tacrolimus treatment. CYP3A5*3 genotypes were detected by PCR-RFLP method and SLCO1B1(rs2306283, rs4149032) genotypes were detected by Agena Bioscience Mass ARRAY® system. Trough concentrations of tacrolimus on day 7 after renal transplantation were collected from clinical data. Correlations between genetic polymorphisms and tacrolimus concentrations were analyzed by SPSS. In CYP3A5 nonexpressers, the dose-adjusted concentration of tacrolimus in SLCO1B1 rs2306283 CC carriers was considerably higher than that in CT and TT carriers. The results illustrated that SLCO1B1 rs2306283 polymorphisms were associated with tacrolimus concentrations, and genotyping for this SNP may be useful for individualized medicine of tacrolimus.
本研究旨在探讨中国肾移植受者中溶质载体有机阴离子转运体家族成员1B1（SLCO1B1）基因多态性与他克莫司血药浓度之间的关系。收集了89例接受他克莫司治疗的肾移植受者的血液样本和临床资料。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测细胞色素P450 3A5（CYP3A5）*3基因型，采用Agena Bioscience Mass ARRAY®系统检测SLCO1B1（rs2306283、rs4149032）基因型。从临床资料中收集肾移植术后第7天他克莫司的谷浓度。运用SPSS软件分析基因多态性与他克莫司浓度之间的相关性。在CYP3A5非表达者中，SLCO1B1 rs2306283基因CC型携带者的他克莫司剂量校正浓度显著高于CT型和TT型携带者。结果表明，SLCO1B1 rs2306283基因多态性与他克莫司浓度有关，对该单核苷酸多态性（SNP）进行基因分型可能有助于他克莫司的个体化用药。
