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辐照深冻异种半月板组织具有软骨保护作用，可实现半月板全切除后再生。

The Radiated Deep-frozen Xenogenic Meniscal Tissue Regenerated the Total Meniscus with Chondroprotection.

机构信息

Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, 100191, P. R. China.

Department of Orthopaedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, P. R. China.

出版信息

Sci Rep. 2018 Jun 13;8(1):9041. doi: 10.1038/s41598-018-27016-w.

DOI:10.1038/s41598-018-27016-w
PMID:29899552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5998046/
Abstract

Meniscal allograft transplantation yields good and excellent results but is limited by donor availability. The purpose of the study was to evaluate the effectiveness of radiated deep-frozen xenogenic meniscal tissue (RDF-X) as an alternative graft choice in meniscal transplantation. The xenogenic meniscal tissues were harvested from the inner 1/3 part of the porcine meniscus and then irradiated and deeply frozen. The medial menisci of rabbits were replaced by the RDF-X. Meniscal allograft transplantation, meniscectomy and sham operation served as controls. Only a particular kind of rabbit-anti-pig antibody (molecular ranging 60-80 kD) was detected in the blood serum at week 2. The menisci of the group RDF-X grossly resembled the native tissue and the allograft meniscus with fibrocartilage regeneration at postoperative 1 year. Cell incorporation and the extracellular matrix were mostly observed at the surface and the inner 1/3 part of the newly regenerated RDF-X, which was different from the allograft. The biomechanical properties of the group RDF-X were also approximate to those of the native meniscus except for the compressive creep. In addition, chondroprotection was achieved after the RDF-X transplantation although the joint degeneration was not completely prevented. To conclude, the RDF-X could be a promising alternative for meniscal transplantation with similar tissue regeneration capacity to allograft transplantation and superior chondroprotection. The potential minor immunological rejection should be further studied before its clinical application.

摘要

异体半月板移植可取得良好和优秀的效果，但受到供体可用性的限制。本研究旨在评估辐照深冻异种半月板组织（RDF-X）作为半月板移植替代移植物的效果。从猪半月板的内 1/3 部分收获异种半月板组织，然后进行辐照和深冷冻。用 RDF-X 替代兔内侧半月板。半月板同种异体移植、半月板切除术和假手术作为对照。仅在第 2 周时在血清中检测到一种特殊的兔抗猪抗体（分子量为 60-80kD）。RDF-X 组的半月板大体上与天然组织和同种异体半月板相似，在术后 1 年时出现纤维软骨再生。细胞掺入和细胞外基质主要观察到在新再生的 RDF-X 的表面和内 1/3 部分，这与同种异体半月板不同。RDF-X 组的生物力学性能也与天然半月板相近，除了压缩蠕变。此外，尽管不能完全预防关节退变，但在 RDF-X 移植后仍能实现软骨保护。结论，RDF-X 可能是一种有前途的半月板移植替代物，具有与同种异体移植相似的组织再生能力和更好的软骨保护作用。在其临床应用之前，应进一步研究潜在的轻微免疫排斥反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/c01bf7691364/41598_2018_27016_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/759209c1f4bb/41598_2018_27016_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/c01bf7691364/41598_2018_27016_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/1ec47c77cabe/41598_2018_27016_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/ebe71a4d8010/41598_2018_27016_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/bbe919291aca/41598_2018_27016_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/788426c3eff8/41598_2018_27016_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/a678692e4723/41598_2018_27016_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/af7a264ccc8d/41598_2018_27016_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/51e0a7ac3463/41598_2018_27016_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/12dd3d96805c/41598_2018_27016_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/759209c1f4bb/41598_2018_27016_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/668d/5998046/c01bf7691364/41598_2018_27016_Fig10_HTML.jpg

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