Department of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China.
Department of Pathology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Oncol Rep. 2018 Aug;40(2):759-766. doi: 10.3892/or.2018.6496. Epub 2018 Jun 14.
Trop2 is considered to have an important function in tumor metastasis and the promotion of epithelial‑mesenchymal transition (EMT). E‑cadherin is a crucial factor in intercellular adhesion and EMT transformation. In the present study, we detected the expression of Trop2 and E‑cadherin in breast cancer (BC) to better define their prognostic value. The mRNA expression levels of these two genes in 20 cases of fresh BC tissues were detected by quantitative real‑time polymerase chain reaction (qRT‑PCR). We also detected the expression levels of these two genes by immunohistochemistry (IHC) in 312 BC tissues, and the correlations between the expression of these two genes and the clinicopathological characteristics in BC patients were analyzed. The mRNA and protein expression levels of the two genes in BC cell lines were studied by qRT‑PCR and western blotting. The results indicated that Trop2+/E‑cadherin‑ was expressed in BC tissues more than that in the matched adjacent tissues. The protein expression results obtained via IHC were similar to the mRNA expression results. Trop2+/E‑cadherin‑ that was expressed in BC was associated with lymph node status, metastasis, tumor‑node‑metastasis (TNM) stage, and ER‑/PR‑/HER2‑ expression. BC patients that expressed Trop2+/E‑cadherin‑ had poor overall survival rates. The results of Trop2 and E‑cadherin expression levels obtained in the BC cell lines were the same as those obtained in the BC tissues. Overall, Trop2 has a potential role in the promotion of EMT in BC and it could be considered as a therapeutic target in the future.
Trop2 被认为在肿瘤转移和上皮-间质转化（EMT）的促进中具有重要功能。E-钙黏蛋白是细胞间黏附的关键因素，也是 EMT 转化的关键因素。在本研究中，我们检测了乳腺癌（BC）中 Trop2 和 E-钙黏蛋白的表达，以更好地定义它们的预后价值。通过实时定量聚合酶链反应（qRT-PCR）检测 20 例新鲜 BC 组织中这两个基因的 mRNA 表达水平。我们还通过免疫组织化学（IHC）检测了 312 例 BC 组织中这两个基因的表达水平，并分析了这两个基因的表达与 BC 患者临床病理特征之间的相关性。通过 qRT-PCR 和 Western blot 研究了这两个基因在 BC 细胞系中的 mRNA 和蛋白表达水平。结果表明，Trop2+/E-钙黏蛋白-在 BC 组织中的表达高于配对的相邻组织。IHC 获得的蛋白表达结果与 mRNA 表达结果相似。在 BC 中表达的 Trop2+/E-钙黏蛋白-与淋巴结状态、转移、肿瘤-淋巴结-转移（TNM）分期和 ER-/PR-/HER2-表达有关。表达 Trop2+/E-钙黏蛋白-的 BC 患者总生存率较差。BC 细胞系中 Trop2 和 E-钙黏蛋白表达水平的结果与 BC 组织中的结果相同。总之，Trop2 在促进 BC 中的 EMT 中具有潜在作用，将来可能被视为一种治疗靶点。
