Cho M I, Garant P R
Acta Anat (Basel). 1985;121(4):205-15. doi: 10.1159/000145968.
The administration of colchicine disrupts the normal organization of the Golgi complex and blocks the secretion of collagen precursors in periodontal ligament fibroblasts of the mouse. The fate of the unsecreted collagen precursors contained in Golgi-derived saccules and newly formed dense bodies was followed by electron microscopy. A progressive condensation of saccule content along with phase separation of electron-dense and electron-lucent material was observed. Fusion of saccules with dense secretory bodies gave rise to larger inclusions (zebra bodies; ZB) filled with a combination of electron-dense and electron-lucent material. In some ZB, these materials appeared to polymerize into fibrillar units. The fibrillar units stained with silver methenamine like normal collagenous fibrils. These results suggest that unsecreted collagen precursors accumulate in vesicular compartments within which partial polymerization can occur. This finding may explain some reports of intracellular collagenous fibrils in fibroblasts of pathologically altered connective tissues.
秋水仙碱的施用会破坏高尔基体的正常组织结构，并阻断小鼠牙周膜成纤维细胞中胶原前体的分泌。通过电子显微镜追踪高尔基体衍生的囊泡和新形成的致密小体中未分泌的胶原前体的命运。观察到囊泡内容物逐渐浓缩，同时电子致密和电子透亮物质发生相分离。囊泡与致密分泌小体融合形成了更大的包含物（斑马小体；ZB），其中填充有电子致密和电子透亮物质的混合物。在一些ZB中，这些物质似乎聚合成纤维状单元。这些纤维状单元用亚甲胺银染色，类似于正常的胶原纤维。这些结果表明，未分泌的胶原前体在囊泡隔室内积累，在其中可以发生部分聚合。这一发现可能解释了一些关于病理改变的结缔组织成纤维细胞中细胞内胶原纤维的报道。
