Luo Xu, Zeng Taofang, He Sunyue, Lin Cai
Center of the Wound and Burn, the First Affiliated Hospital of Wenzhou Medical University, China.
Department of Burn, the People's Hospital of Yichun, China.
J Burn Care Res. 2018 Jun 13;39(4):481-490. doi: 10.1093/jbcr/irx002.
We investigated the effects of microporous porcine acellular dermal matrices (MPADM)-containing bone marrow-derived mesenchymal stem cells (BMMSCs) on accessory skin cell regeneration in vivo. Two kinds of the porcine acellular dermal matrices were prepared: one with microsized pores (the MPADM) and another without pores (the PADM). BMMSC populations from a Sprague-Dawley (SD) rat were seeded on both PADMs and MPADMs and cultured in vitro for 5 days. These rats were randomly divided into four groups: BMMSCs on an MPADM and covered with a PADM layer (group A), MPADM without cells and covered with a split-thickness skin graft (SSG) (group B), BMMSCs on an MPADM and covered with an SSG (group C), and BMMSCs on a PADM and covered with an SSG (group D). On post-surgery day (PSD) 5, all groups survived, except for group D. On PSD 7, there was no significant difference in the functional vascularization between groups A, B, and C. On PSD 14, large quantities of new capillaries, a larger rough endoplasmic reticulum in fibroblasts, and de novo unmyelinated nerve endings could be observed at the junction between the skin graft and the dermal matrix in group C; however, these structures were absent in groups A and B. The experimental results showed that MPADM could induce exogenous differentiation of BMMSCs in vivo and promote reconstruction of skin accessory cells.
我们研究了含有骨髓间充质干细胞（BMMSCs）的微孔猪脱细胞真皮基质（MPADM）对体内附属皮肤细胞再生的影响。制备了两种猪脱细胞真皮基质：一种具有微孔（MPADM），另一种无孔（PADM）。将来自Sprague-Dawley（SD）大鼠的BMMSC群体接种在PADM和MPADM上，并在体外培养5天。将这些大鼠随机分为四组：BMMSCs接种在MPADM上并覆盖一层PADM（A组），无细胞的MPADM并覆盖断层皮片（SSG）（B组），BMMSCs接种在MPADM上并覆盖SSG（C组），以及BMMSCs接种在PADM上并覆盖SSG（D组）。术后第5天（PSD 5），除D组外，所有组均存活。在PSD 7时，A、B和C组之间的功能性血管化无显著差异。在PSD 14时，在C组的皮肤移植片与真皮基质的交界处可观察到大量新的毛细血管、成纤维细胞中较大的粗面内质网和新生的无髓神经末梢；然而，A组和B组中没有这些结构。实验结果表明，MPADM可在体内诱导BMMSCs的外源性分化，并促进皮肤附属细胞的重建。
