Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, P. R. China.
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, P. R. China.
J Basic Microbiol. 2018 Aug;58(8):686-697. doi: 10.1002/jobm.201800100. Epub 2018 Jun 14.
The induced resistance against plant pathogens via biocontrol agents is considered as an eco-friendly and promising strategy. In this study, the induced resistance against Magnaporthe oryzae (M. oryzae) in rice seedling by a new potential biocontrol agent Streptomyces JD211 (JD211) was evaluated. The effects of JD211 on defense-related enzymes activities and defense genes expression were investigated. The biocontrol efficacy of different JD211 concentrations was different, and the treatment of 10 g kg JD211 achieved the highest biocontrol efficacy. Activities of catalase, phenylalanine ammonia-lyase (PAL) and β-1,3-glucanase significantly increased in the presence of JD211. The gene expression level of both PAL and pathogenesis related protein 1 increased when rice seedlings were inoculated with JD211 alone or co-inoculated with M. oryzae, and the expression level of chitinase gene was enhanced by JD211 in the later stage. All results suggested that JD211 could increase the rice resistance by stimulating a series of defense responses, which was the result of induced systemic resistance by JD211. This work will provide a new biocontrol agent against Magnaporthe oryzae in rice seedling.
通过生物防治剂诱导植物病原体抗性被认为是一种环保且有前景的策略。本研究评估了新型潜在生物防治剂链霉菌 JD211（JD211）诱导水稻幼苗对稻瘟病菌（M. oryzae）抗性的能力。研究了 JD211 对防御相关酶活性和防御基因表达的影响。不同 JD211 浓度的生物防治效果不同，10g/kg JD211 的处理效果最佳。CAT、PAL 和β-1,3-葡聚糖酶的活性在 JD211 存在下显著增加。当单独接种 JD211 或与稻瘟病菌共接种时，PAL 和病程相关蛋白 1 的基因表达水平均升高，几丁质酶基因的表达水平在后期也被 JD211 增强。所有结果表明，JD211 通过刺激一系列防御反应来提高水稻的抗性，这是 JD211 诱导系统抗性的结果。这项工作将为水稻幼苗提供一种新的稻瘟病菌生物防治剂。
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