Kaari Manigundan, Joseph Jerrine, Manikkam Radhakrishnan, Sreenivasan Ayswarya, Venugopal Gopikrishnan, Alexander Balamurugan, Krishnan Sakthivel
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu 600 119 India.
Department of Plant Pathology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu 641 003 India.
Indian J Microbiol. 2022 Mar;62(1):32-39. doi: 10.1007/s12088-021-00963-1. Epub 2021 Jul 26.
Biological control of phytopathogen is a promising approach when compared to the use of chemical agents. In the present study, seven cultures showing promising anti biofilm activity against was mixed individually with farmyard manure. All the fortified farmyard manure (SFYM) were screened for plant growth promotion and control of bacterial wilt caused by on tomato Further, the ability of SFYM on stimulating the production of defense-related enzymes in -inoculated tomato plants was investigated. When compared to the control tomato plants, the SFYM-treated plants had longer shoot and root length along with higher fresh and dry weight. The maximum level of chlorophyll was observed in the plants treated with strain UP1A-1 (2.21 ± 0.18 mg g). Strain UP1A-1 also showed maximum of 96.8 ± 1.4% biocontrol efficacy in tomato plants challenged with . In addition, the UP1A-1 treated tomato plants showed maximum accumulation of total phenolics (3.02 ± 0.09 mg g) after 6 days of pathogen inoculation (DPI). Similarly, tomato plants treated with UP1A-1 showed highest level of peroxides, polyphenol oxidase and phenylalanine ammonia lyase during 1-9 DPI. Findings of present study revealed that the culture UP1A-1 fortified farm yard manure could be applied as an eco-friendly alternative to synthetic agents for controlling bacterial wilt in tomato plants.
与使用化学药剂相比，对植物病原体进行生物防治是一种很有前景的方法。在本研究中，将七种对[未提及的病原体]显示出有前景的抗生物膜活性的培养物分别与农家肥混合。对所有强化农家肥（SFYM）进行了促进植物生长和防治番茄青枯病的筛选。此外，还研究了SFYM对激发接种[未提及的病原体]的番茄植株中防御相关酶产生的能力。与对照番茄植株相比，经SFYM处理的植株茎和根更长，鲜重和干重更高。在用菌株UP1A - 1处理的植株中观察到叶绿素的最高含量（2.21±0.18毫克/克）。菌株UP1A - 1在受[未提及的病原体]侵染的番茄植株中也显示出最高达96.8±1.4%的生物防治效果。此外，在病原体接种6天后（DPI），经UP1A - 1处理的番茄植株中总酚类物质的积累量最高（3.02±0.09毫克/克）。同样，在接种后1 - 9天内，经UP1A - 1处理的番茄植株中过氧化物、多酚氧化酶和苯丙氨酸解氨酶的水平最高。本研究结果表明，用菌株UP1A - 1强化的农家肥可作为一种生态友好型替代品，用于防治番茄植株的青枯病，替代合成药剂。
