设计和评估巢式 PCR 引物用于特异性检测牡蛎中的基因 I 组诺如病毒。
Design and evaluation of nested PCR primers for specific detection of genogroup I noroviruses in oysters.
机构信息
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China.
出版信息
Mol Cell Probes. 2018 Aug;40:40-43. doi: 10.1016/j.mcp.2018.06.002. Epub 2018 Jun 11.
A pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters.
根据公共数据库中所有 GI 序列，设计了一对用于基因组 I（GI）诺如病毒的嵌套 PCR 通用引物（NGIOF 和 NGIOR）。评估了这些引物的特异性、灵敏度和覆盖度，结果表明它们在贝类中检测 GI 诺如病毒时具有可靠的性能。
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