Fan Fang-Fang, Guo Fei-Fei, Zhang Wen, Zhang Yi, Yang Hong-Jun, Zhang Yi, Li De-Feng, Zhang Jing, Zhang Jing-Jing
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 111137, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(9):1789-1797. doi: 10.19540/j.cnki.cjcmm.20180125.011.
In this study, a computer-based network pharmacology approach was applied to investigate the potential mechanism and important components of Rhodiola crenulata in the protection of H9c2 cells against hydrogen peroxide (H₂O₂)-induced oxidative stress. The intestinal absorption liquid of R. crenulata enhanced the cell viability, maintained cell morphology and inhibited cell apoptosis in the H₂O₂-induced oxidative stress in H9c2. Then, computer-based network pharmacology was used to analyze the relevant mechanism. A total of 133 oxidative stress-related compounds were screened out; and 26 of them occupied the top 20%, and all of the compounds enriched in 43 oxidative stress-related key targets. Finally, a "compound-target-pathway-function" network was constructed. Based on the analysis of the network pharmacology, R. crenulata protected H9c2 cells against H₂O₂-induced oxidative stress probably by affecting apoptosis-related processes, such as cell death, nitric oxide metabolism, oxidative stress, mitochondrial mechanism, redox process, redox-related enzyme activty and other oxidative stress-related process. And salidroside, ethyl gallate and catechins, which were the main components of R. crenulata, played an important role in this process. Therefore, the potential mechanism and important components of R. crenulata revealed the protective effect on oxidative stress. This study shows a multi-component, multi-target and overall regulation effect of R. crenulata on the oxidative stress, and provides a reliable reference for subsequent systematic experimental studies for the pharmacodynamic material foundation and mechanism of action R. crenulata.
在本研究中，应用基于计算机的网络药理学方法来研究大花红景天在保护H9c2细胞免受过氧化氢（H₂O₂）诱导的氧化应激中的潜在机制和重要成分。大花红景天的肠道吸收液在H₂O₂诱导的H9c2氧化应激中提高了细胞活力，维持了细胞形态并抑制了细胞凋亡。然后，使用基于计算机的网络药理学来分析相关机制。共筛选出133种与氧化应激相关的化合物；其中26种占前20%，所有化合物富集于43个与氧化应激相关的关键靶点。最后，构建了一个“化合物-靶点-途径-功能”网络。基于网络药理学分析，大花红景天可能通过影响凋亡相关过程，如细胞死亡、一氧化氮代谢、氧化应激、线粒体机制、氧化还原过程、氧化还原相关酶活性及其他氧化应激相关过程，来保护H9c2细胞免受H₂O₂诱导的氧化应激。而大花红景天的主要成分红景天苷、没食子酸乙酯和儿茶素在这一过程中发挥了重要作用。因此，大花红景天的潜在机制和重要成分揭示了其对氧化应激的保护作用。本研究表明大花红景天对氧化应激具有多成分、多靶点和整体调节作用，为后续关于大花红景天药效物质基础和作用机制的系统实验研究提供了可靠参考。
