Xu Rong-Rong, Liu Hao-Long, Chen Xiao-Qing, Ba Yin-Ying, Wang Xing, Wu Xia
Beijing Key Lab of Traditional Chinese Medicine, Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing Institute for Drug Control, Beijing 100035, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(9):1798-1805. doi: 10.19540/j.cnki.cjcmm.20180305.003.
The metabolites were detected in feces and urine of rats orally administrated alkaloids of Piper longum by using high performance liquid chromatography coupled with a Fourier Transform ion cyclotron resonance mass spectrometer (HPLC-FT-MS). According to the mass spectrometric data and reported literature, the structures of metabolites were identified. Several metabolites were analyzed and belonged to piperine, piperanine, piperlonguminine, Δα,β-dihydropiperlonguminine and pellitorine, respectively. The metabolites of alkaloids from P. longum alkaloids were produced through Ⅰ phase and Ⅱ phase metabolism reaction, and were excreted with urination and defecation. The approach provided a rapid method for characterizing the metabolites of P. longum alkaloids and gave the truly active structures and the action mechanism of their neuroprotective effects.
采用高效液相色谱-傅里叶变换离子回旋共振质谱联用仪（HPLC-FT-MS），在经口给予大鼠荜茇生物碱的粪便和尿液中检测到了代谢产物。根据质谱数据和已报道的文献，鉴定了代谢产物的结构。分析了几种代谢产物，它们分别属于胡椒碱、胡椒酰胺、荜茇明宁碱、Δα,β-二氢荜茇明宁碱和异胡椒酰胺。荜茇生物碱的代谢产物是通过Ⅰ相和Ⅱ相代谢反应产生的，并通过排尿和排便排出体外。该方法为表征荜茇生物碱的代谢产物提供了一种快速方法，并给出了其神经保护作用的真正活性结构和作用机制。
