[Determination of bisphenol A in human blood by using fast phospholipid removal solid phase extraction method-ultra performance liquid chromatography tandem mass spectrometry].

OBJECTIVE

To develop a method for determination of bisphenol A in human blood by fast phospholipid removal solid phase extraction method-ultra performance liquid chromatography tandem mass spectrometry( UPLC-MS/MS).

METHODS

Enzymatic hydrolysis( β-glucuronidase/arylsulfatase) and phospholipid removal solid phase extraction were used to treat the blood samples under the acidic condition, a Infinity Lab Poroshell 120 PFP column( 100 mm × 3. 0 mm, 2. 7 μm) was used for LC separation, ESI negative ion scan was used with multiple reaction monitoring( MRM) mode.

RESULTS

The calibration curve was linear in the range of 0. 1-100 ng/mL for bisphenol A with correlation coefficients more than 0. 999. The limit of detection was 0. 05 ng/mL, the limit of quantity was 0. 15 ng/mL. The recoveries of the method for bisphenol A at three spiked levels of 0. 5, 5 and 50 ng/mL ranged from 87. 3%to 112. 1%. The relative standard deviation( RSD) of intra and inter day were range from3. 3%-8. 2%, 4. 9%-10. 7%( n = 6), respectively.

CONCLUSION

The method is successfully applied in the analysis of bisphenol A in human blood with its simple operation, sensitivity and accuracy.