Ma Baotao, Yuan Xiaoyan, Wang Xiaodan, Gao Peng, Zhao Zengming, Yang Jianjun, Peng Shuangqing, Xu Haibin
School of Public Health and Management, Ningxia Medical University , Yinchuan 750004, China.
Wei Sheng Yan Jiu. 2017 May;46(3):443-454.
To evaluate the food safety of recombinant human lactoferrin( rhLF) and its iron nutrition.
100 SPF grade weanling SD rats with half male and female, were randomly divided into five groups and they were control group, 100 and200 g/kg bLF group, 100 and 200 g/kg rhLF group. Rates in control group were feed with AIN-93 laboratory rodent pure fodder, while bLF and rhLF group were feed with sample protein part and all alternative formulations existed casein component respectively. All Rates were free of water consumption and observed in 28 days. Body weight, food consumption, food utilization and organ coefficient were recorded, while hematology, iron nutrition index [serum iron( SI), total iron binding capacity( TIBC), Transferringsaturation( TS), Soluble transferrin receptor( s TfR) ] and clinical biochemistry were measured.
There was no significant difference in body weight between 200 g/kg bLF group and 200 g/kg rhLF groups neither for male nor female animals, while both significantly lower than the control group( P < 0. 05). No significant change in clinical biochemistry hematology, anatomy and histopathology results were observed for 100 g/kg and 200 g/kg rhLF group. There was no significant difference in serum iron level and transferrin saturation between bLF group and rhLF group, but the serum iron level and transferrin saturation of both bLF group and rhLF group were higher than the control group.
No significant difference were observed in food safety indicators and iron nutrition improvement between 28-day rhLF and bLF in the present study.
评估重组人乳铁蛋白（rhLF）的食品安全及其铁营养状况。
选取100只SPF级断乳SD大鼠，雌雄各半，随机分为五组，即对照组、100和200 g/kg牛乳铁蛋白（bLF）组、100和200 g/kg rhLF组。对照组大鼠饲喂AIN-93实验啮齿动物纯饲料，而bLF组和rhLF组分别饲喂样品蛋白部分且所有替代配方均含有酪蛋白成分。所有大鼠自由饮水，观察28天。记录体重、食物摄入量、食物利用率和器官系数，同时测定血液学指标、铁营养指标[血清铁（SI）、总铁结合力（TIBC）、转铁蛋白饱和度（TS）、可溶性转铁蛋白受体（sTfR）]和临床生化指标。
200 g/kg bLF组和200 g/kg rhLF组雌雄动物体重均无显著差异，但均显著低于对照组（P<0.05）。100 g/kg和200 g/kg rhLF组临床生化、血液学、解剖学和组织病理学结果均无显著变化。bLF组和rhLF组血清铁水平和转铁蛋白饱和度无显著差异，但bLF组和rhLF组血清铁水平和转铁蛋白饱和度均高于对照组。
本研究中，28天的rhLF和bLF在食品安全指标和铁营养改善方面未观察到显著差异。
