用于大肠杆菌、恶臭假单胞菌和铜绿假单胞菌的穿梭载体。

Shuttle vector for Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa.

作者信息

Lushnikov A A, Andreevskaya E A, Lukashova L I, Lobach J B, Domaradskij I V

出版信息

Basic Life Sci. 1985;30:657-62. doi: 10.1007/978-1-4613-2447-8_45.

Abstract

A hybrid plasmid capable of replication in 2 different genera, Escherichia and Pseudomonas, was constructed. This plasmid DNA can be used as a cloning vector in E. coli and pseudomonades cells. The described hybrid plasmid pLD411 has been constructed on the basis of 2 small E. coli vector R-plasmids used in our laboratory and cryptic plasmid pWW2 or P. putida MT1. Plasmid pLD411 DNA was mapped with restrictases; its biological activity in transformations of different bacterial strains was studied, and the characteristics of transformed cells were also described.

摘要

构建了一种能够在大肠杆菌和假单胞菌这两个不同属中复制的杂种质粒。这种质粒DNA可作为大肠杆菌和假单胞菌细胞中的克隆载体。所描述的杂种质粒pLD411是基于我们实验室使用的两种小的大肠杆菌载体R质粒以及隐蔽质粒pWW2或恶臭假单胞菌MT1构建的。用限制酶对质粒pLD411 DNA进行了图谱分析；研究了其在不同细菌菌株转化中的生物学活性，并描述了转化细胞的特性。