Ren Haiwei, Sun Renlian, Wang Jian
Department of Dialysis, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China.
Department of Nephrology, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China.
Exp Ther Med. 2018 Jun;15(6):5183-5188. doi: 10.3892/etm.2018.5857. Epub 2018 Feb 12.
The relationship of melatonin (MT) level, oxidative stress and inflammatory status with osteoporosis in the maintenance hemodialysis (MHD) of chronic renal failure was investigated. Ninety-four patients with chronic renal failure treated in Dezhou People's Hospital (Dezhoo, China) and receiving MHD for more than 3 months from May to April 2017 were selected and divided into the osteoporosis group (observation group, n=49) and the non-osteoporosis group (control group, n=45) based on whether osteoporosis was involved. The serum advanced oxidation protein products (AOPP), MT level and inflammatory factors [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1] in patients were detected via enzyme-linked immunosorbent assay (ELISA). Moreover, the malondialdehyde (MDA) level in serum was detected via thibabituric acid (TBA) assay and the spinal bone mineral density (BMD) was detected using the total BMD testing instrument, followed by correlation analysis. The MT level in observation group was significantly lower than that in control group, but the levels of AOPP and MDA were significantly higher than those in control group (P<0.05). The levels of inflammatory factors (TNF-α, IL-6 and IL-1) in observation group were obviously higher than those in the control group (P<0.05). Besides, the spinal BMD and T-value in observation group were significantly lower than those in the control group (P<0.05). Pearson's correlation analysis showed that AOPP, MDA, TNF-α, IL-6 and IL-1 levels were negatively correlated with BMD, but MT was positively correlated with BMD (P<0.05). Osteoporosis occurs easily in MHD patients; oxidative stress and inflammatory degree are negatively correlated with osteoporosis, but MT is positively correlated with osteoporosis.
研究了褪黑素（MT）水平、氧化应激和炎症状态与慢性肾衰竭维持性血液透析（MHD）患者骨质疏松症的关系。选取2017年5月至2018年4月在德州市人民医院（中国德州）接受MHD治疗3个月以上的94例慢性肾衰竭患者，根据是否患有骨质疏松症分为骨质疏松组（观察组，n = 49）和非骨质疏松组（对照组，n = 45）。采用酶联免疫吸附测定（ELISA）法检测患者血清晚期氧化蛋白产物（AOPP）、MT水平及炎症因子[肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和IL-1]。此外，采用硫代巴比妥酸（TBA）法检测血清丙二醛（MDA）水平，使用总骨密度测试仪检测脊柱骨密度（BMD），随后进行相关性分析。观察组MT水平显著低于对照组，而AOPP和MDA水平显著高于对照组（P < 0.05）。观察组炎症因子（TNF-α、IL-6和IL-1）水平明显高于对照组（P < 0.05）。此外，观察组脊柱BMD和T值显著低于对照组（P < 0.05）。Pearson相关性分析显示，AOPP、MDA、TNF-α、IL-6和IL-1水平与BMD呈负相关，而MT与BMD呈正相关（P < 0.05）。MHD患者易发生骨质疏松症；氧化应激和炎症程度与骨质疏松症呈负相关，而MT与骨质疏松症呈正相关。
