Ambudkar S V, Maloney P C
Biochem Biophys Res Commun. 1985 Jun 14;129(2):568-75. doi: 10.1016/0006-291x(85)90189-5.
Membrane protein solubilized by octyl-beta-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate:sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.
在分散磷脂存在的情况下，用辛基-β-D-葡萄糖苷溶解的膜蛋白与经浴式超声处理的脂质体和额外的去污剂一起孵育。稀释时形成的蛋白脂质体表现出与磷酸：糖6-磷酸反向转运体重构相一致的转运和交换特性。因此，仅当使用来自诱导细胞的蛋白质时才发现磷酸自我交换；这种交换被糖6-磷酸阻断，而不是被糖1-磷酸阻断；并且蛋白脂质体在没有添加能量来源的情况下支持2-脱氧葡萄糖6-磷酸的积累。当在革兰氏阳性磷脂存在的情况下进行时，蛋白质的溶解和重构最为有效。
