Jeffrey P L, Leung W N, Rostas J A
Biochem Int. 1985 May;10(5):715-22.
This study evaluates the relative contributions of neural impulse activity and neurotrophic (non-impulse) factors by comparing the effects of denervation and the blockage of neural impulse activity on various biochemical parameters of mixed muscle sarcolemma. Muscle paralysis was induced by repeated injection of Tetrodotoxin (TTx) to the sciatic nerve. After seven days of inactivity the isolated sarcolemma was analyzed for protein and glycoprotein composition, Na+/K+ ATPase activity, Concanavalin A binding to intact sarcolemma and to carbohydrate components separated in SDS-polyacrylamide gels and sialyl and galactosyl transferase activity. The results have shown that following muscle inactivity all of the parameters except glycosyl transferases changed in a similar manner but to a lesser degree than denervation. It is concluded that trophic factors in addition to neural impulse activity play a role in the regulation of a number of surface membrane properties.
本研究通过比较去神经支配和神经冲动活动阻断对混合肌肌膜各种生化参数的影响，评估神经冲动活动和神经营养（非冲动）因子的相对贡献。通过向坐骨神经反复注射河豚毒素（TTx）诱导肌肉麻痹。在不活动7天后，分析分离的肌膜的蛋白质和糖蛋白组成、Na+/K+ ATP酶活性、伴刀豆球蛋白A与完整肌膜以及与在SDS-聚丙烯酰胺凝胶中分离的碳水化合物成分的结合情况，以及唾液酸基转移酶和半乳糖基转移酶活性。结果表明，肌肉不活动后，除糖基转移酶外，所有参数的变化方式相似，但程度小于去神经支配。得出的结论是，除神经冲动活动外，营养因子在多种表面膜特性的调节中起作用。
