Driessen M, Weitz G, Brouwer-Kelder E M, Donker-Koopman W E, Bastiaannet J, Sandhoff K, Barranger J A, Tager J M, Schram A W
Biochim Biophys Acta. 1985 Jul 26;841(1):97-102. doi: 10.1016/0304-4165(85)90278-8.
Antibodies raised against the soluble form of acid sphingomyelinase from human urine and placenta are able to precipitate about 70% of the sphingomyelinase activity present in preparations of urinary sphingomyelinase. In contrast, no precipitation of sphingomyelinase activity occurs in detergent-containing preparations from placenta or splenic membranes. The formation of immune complexes between the antibodies and urinary sphingomyelinase is inhibited if detergents are added. With the non-ionic detergent Triton X-100 significant inhibition occurs only above the critical micellar concentration of the detergent. With the anionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulphonate (Chaps) substantial inhibition is already observed below the critical micellar concentration of the detergent.
针对人尿和胎盘来源的可溶性酸性鞘磷脂酶产生的抗体，能够沉淀尿鞘磷脂酶制剂中约70%的鞘磷脂酶活性。相比之下，胎盘或脾膜含去污剂的制剂中未出现鞘磷脂酶活性的沉淀。如果添加去污剂，抗体与尿鞘磷脂酶之间免疫复合物的形成会受到抑制。对于非离子去污剂Triton X-100，只有在去污剂的临界胶束浓度以上才会出现显著抑制。对于阴离子去污剂3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐（Chaps），在低于去污剂的临界胶束浓度时就已观察到显著抑制。
