Opt Lett. 2018 Jun 15;43(12):2973-2976. doi: 10.1364/OL.43.002973.
Half-circle illumination-based differential phase contrast (DPC) microscopy has been utilized to recover phase images through a pair of images along multiple axes. Recently, the half-circle based DPC using 12-axis measurements significantly provides a circularly symmetric phase transfer function to improve accuracy for more stable phase recovery. Instead of using half-circle-based DPC, we propose a new scheme of DPC under radially asymmetric illumination to achieve circularly symmetric phase transfer function and enhance the accuracy of phase recovery in a more stable and efficient fashion. We present the design, implementation, and experimental image data demonstrating the ability of our method to obtain quantitative phase images of microspheres, as well as live fibroblast cell samples.
基于半环形照明的微分相衬（DPC）显微镜已被用于通过一对多轴图像来恢复相位图像。最近，基于半环形的 DPC 使用 12 轴测量显著提供了一个圆对称的相位传递函数，以提高更稳定的相位恢复的准确性。我们提出了一种新的基于径向非对称照明的 DPC 方案，以实现圆对称的相位传递函数，并以更稳定和高效的方式提高相位恢复的准确性。我们介绍了该方法的设计、实现和实验图像数据，证明了该方法能够获取微球和活成纤维细胞样本的定量相位图像的能力。
