Schmidt Nadine, Luhmann Tim, Hüther Liane, Meyer Ulrich, Barth Stefanie A, Geue Lutz, Menge Christian, Frahm Jana, Dänicke Sven
Institute of Molecular Pathogenesis, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Jena, Germany.
Institute of Animal Nutrition, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Brunswick, Germany.
J Anim Physiol Anim Nutr (Berl). 2018 Oct;102(5):1167-1180. doi: 10.1111/jpn.12926. Epub 2018 Jun 15.
Vitamin E (vit E), an essential antioxidant for maintaining the stability of biological membranes and the function of the immune system, is considered to support adaptive immune responses and performance in cattle. The principal virulence factor of Shiga toxin (Stx)-producing Escherichia coli (STEC), the eponymous Stx, modulates cellular immune responses in cattle, the primary STEC reservoir. Active and passive immunization of calves with Shiga toxoids (rStx ) was recently shown to reduce the STEC shedding. Here, we examined the influence of vit E on calves' serum α-tocopherol, performance, haematology, blood chemistry and its interaction with rStx immunization. Data from calves having received passive (colostrum from immunized cows) and active (intramuscularly at 5th and 8th weeks of life) vaccination with rStx (n = 24) were compared to unvaccinated controls (n = 24; fed with low anti-Stx colostrum, placebo injected). For each vaccination group, data were analysed according to the level of vit E supplementation offered by milk replacer (188 IU all-rac-α-tocopheryl acetate daily [VitE ] vs. 354 IU [VitE ]). An increase by 79% in daily vit E supplementation led to slightly higher serum α-tocopherol level and earlier concentrate intake at the beginning of the experiment without significant differences in live weight gain, haematology, blood chemistry parameters and peripheral CD4 and CD8 T-cell subpopulations. rStx vaccination modulated the CD4 /CD8 ratio irrespective of vit E supplementation but decreased concentrate intake in VitE in a time-dependent manner. Results of our study indicate that an increase in daily vit E supplementation vastly fails to exert effects on laboratory parameters and growth performance. However, observed interactive effects of vit E supply and vaccination on the regulation of feed intake deserves further attention.
维生素E（vit E）是维持生物膜稳定性和免疫系统功能所必需的抗氧化剂，被认为有助于牛的适应性免疫反应和生产性能。产志贺毒素（Stx）的大肠杆菌（STEC）的主要毒力因子，即同名的Stx，可调节牛（主要的STEC宿主）的细胞免疫反应。最近的研究表明，用志贺类毒素（rStx）对犊牛进行主动和被动免疫可减少STEC的排出。在此，我们研究了vit E对犊牛血清α-生育酚、生产性能、血液学、血液生化指标的影响，以及它与rStx免疫的相互作用。将接受rStx被动（来自免疫母牛的初乳）和主动（在生命的第5周和第8周肌肉注射）疫苗接种的犊牛（n = 24）的数据与未接种疫苗的对照组（n = 24；喂食低抗Stx初乳，注射安慰剂）进行比较。对于每个疫苗接种组，根据代乳粉提供的vit E补充水平（每天188 IU全消旋-α-生育酚醋酸酯[VitE ]与354 IU [VitE ]）分析数据。每天vit E补充量增加79%导致实验开始时血清α-生育酚水平略高，精料采食量更早，但在体重增加、血液学、血液生化参数以及外周CD4和CD8 T细胞亚群方面无显著差异。无论vit E补充情况如何，rStx疫苗接种均可调节CD4/CD8比值，但随着时间的推移，VitE组的精料采食量会降低。我们的研究结果表明，每日vit E补充量的增加在很大程度上未能对实验室参数和生长性能产生影响。然而，观察到的vit E供应和疫苗接种对采食量调节的交互作用值得进一步关注。
