Association of Single Nucleotide Polymorphisms of Endothelin, Orexin and Vascular Endothelial Growth Factor Receptor Genes with Obstructive Sleep Apnea among Thai Ethnic.

BACKGROUND

Obstructive sleep apnea (OSA) is a complex disorder characterized by repetitive collapse of upper airway during sleep which strongly influenced by genetic factors, especially those affect regulation of the sleep-wake cycle and endothelial function.

OBJECTIVE

This study investigated the association between single nucleotide polymorphisms (SNPs) in endothelin (EDNRA), orexin (OX1R, OX2R) and vascular endothelial growth factor (VEGFR1) receptor genes with risk of OSA in Thai population.

MATERIAL AND METHOD

All subjects were diagnosed by overnight polysomnography (PSG) before divided into OSA (59) and NOSA (60) groups based on their apnea-hypopnea index (AHI). Serum lipid levels were examined by using enzymatic colorimetric and homogeneous methods. DNAs were extracted and genotyped the SNPs by polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis. Genotype distribution were analyzed using Chi-square test of SPSS program version 15.0.

RESULTS

The triglycerides level of OSA patients was significantly higher than NOSA (p-value = 0.002). The SNPs in EDNRA (rs5335), OX1R (rs2271933), OX2R (rs2292040, rs10456182) and VEGFR1 (rs11149523) genes showed no association with OSA. However, the SNP (rs17675063) in EDNRA gene showed significant differences in genotype distribution in the subjects with and without OSA (p-value = 0.002, odds ratio = 3.29 and 95% CI = 1.86-5.82).

CONCLUSION

Obstructive sleep apnea, Single nucleotide polymorphisms, Endothelin receptor type A, Orexin receptor 1, Orexin receptor 2, Vascular endothelial growth factor receptor type 1.