Kontár Szilvia, Varečka L'udovít, Híreš Michal, Kryštofová Svetlana, Šimkovič Martin
a Institute of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic.
b Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Can J Microbiol. 2018 Nov;64(11):856-864. doi: 10.1139/cjm-2017-0747. Epub 2018 Jun 15.
The effect of light on the binding of Ca to mycelia and to cell walls isolated from aerial mycelia of three strains of Trichoderma spp. was studied. Two independent methods were used to measure the total Ca content in mycelia and the Ca bound to cell walls isolated from aerial mycelia. The results of these methods showed that the light-induced formation and maturation of conidia in Trichoderma spp. is accompanied by increased Ca deposition in mycelia and cell walls. Moreover, the cultivation of Trichoderma atroviride F-534 in the presence of Ca under circadian illumination showed that radioactivity was exclusively localized in the light-induced conidial rings of aerial mycelia. The fluorescence microscopy of chlortetracycline-stained mycelia showed that the major fraction of Ca was accumulated in conidia and fructification structures, or some intracellular compartments in T. atroviride F-534 grown under circadian illumination, while only a limited amount of Ca was associated with hyphal surfaces. In addition, the study of Ca binding to cell walls revealed that T. atroviride F-534 displays both increased Ca binding capacity and elevated affinity to Ca binding upon illumination. The results indicate that conidia formation and (or) maturation is associated with changes in Ca homeostasis.
研究了光照对三种木霉菌株的菌丝体以及从气生菌丝体分离得到的细胞壁中钙结合的影响。采用两种独立的方法来测量菌丝体中的总钙含量以及与从气生菌丝体分离得到的细胞壁结合的钙含量。这些方法的结果表明，木霉菌株中光照诱导的分生孢子形成和成熟伴随着菌丝体和细胞壁中钙沉积的增加。此外，在昼夜光照条件下，在钙存在的情况下培养哈茨木霉F - 534，结果显示放射性仅定位于气生菌丝体的光照诱导分生孢子环中。对金霉素染色的菌丝体进行荧光显微镜观察表明，在昼夜光照条件下生长的哈茨木霉F - 534中，大部分钙积累在分生孢子和结实结构中，或一些细胞内区室中，而只有少量钙与菌丝表面相关。此外，对钙与细胞壁结合的研究表明，光照后哈茨木霉F - 534的钙结合能力增加，对钙结合的亲和力也提高。结果表明，分生孢子形成和（或）成熟与钙稳态的变化有关。
