Lee D, Vetter D, Beatty L, Sadowski P
Can J Biochem Cell Biol. 1985 Apr;63(4):243-8. doi: 10.1139/o85-035.
We have used a plasmid which contains a cloned fragment of T7 DNA to study the properties of general recombination of phage T7 in vitro. It was shown that T7-infected cell extracts promote recombination by the exchange of double strands of DNA. While both products of these double-strand exchanges were detected, we were unable to show that they were formed during a single recombination event.
我们使用了一种含有T7 DNA克隆片段的质粒来研究噬菌体T7在体外的一般重组特性。结果表明，受T7感染的细胞提取物通过DNA双链交换促进重组。虽然检测到了这些双链交换的两种产物，但我们无法证明它们是在单个重组事件中形成的。
