Al-Mudhaffar S A, Rassam M B
Biochem Exp Biol. 1979;15(2):155-65.
The activity of some of the clinically important enzymes was investigated in leukemic sera at 37 degrees, using the Beckman Enzyme Activity Analyzer were found to be slightly elevated in some untreated cases of leukemia (1.), while ALP was found to be frequently elevated. Untreated patients with l. had normal or below normal SCPK activity. The most characteristic and significant rise in activity, was found to be associated with SLDH and SHBDH in most cases of acute l. (86%) and in CML, while any elevation observed in CLL, was very slight. The general kinetic parameters of SLDH and SHBDH, were investigated at 37 degrees in acute leukemic patients. These included optimum substrate concentrations (NADH, pyruvate, and 2-oxobutyrate), the rate of pyruvate and 2-oxobutyrate reduction, substrate-velocity relationship, Km (pyruvate), Km (NADH), Km (2-oxobutyrate) as well as the effect of temperature and pH on the kinetics of the reaction. These kinetic characteristics were found to be differently affected by the leukemic process.
使用贝克曼酶活性分析仪，在37摄氏度下对白血病血清中一些临床重要酶的活性进行了研究。发现在一些未经治疗的白血病病例中，某些酶的活性略有升高（1.），而碱性磷酸酶（ALP）则经常升高。未经治疗的淋巴细胞白血病（l.）患者的肌酸磷酸激酶（SCPK）活性正常或低于正常水平。在大多数急性淋巴细胞白血病（86%）和慢性粒细胞白血病（CML）病例中，发现活性最具特征性和显著性的升高与乳酸脱氢酶（SLDH）和α-羟丁酸脱氢酶（SHBDH）有关，而在慢性淋巴细胞白血病（CLL）中观察到的任何升高都非常轻微。在37摄氏度下对急性白血病患者的SLDH和SHBDH的一般动力学参数进行了研究。这些参数包括最佳底物浓度（烟酰胺腺嘌呤二核苷酸（NADH）、丙酮酸和2-氧代丁酸）、丙酮酸和2-氧代丁酸还原速率、底物-速度关系、丙酮酸的米氏常数（Km（丙酮酸））、NADH的米氏常数（Km（NADH））、2-氧代丁酸的米氏常数（Km（2-氧代丁酸））以及温度和pH对反应动力学的影响。发现这些动力学特征受白血病过程的影响不同。
