Brito Danielle C C, Domingues Sheyla F S, Rodrigues Ana Paula R, Figueiredo José R, Santos Regiane R, Pieczarka Júlio C
Laboratory of Cytogenetics, Centre for Advanced Studies on Biodiversity (CEABIO), Biological Sciences Institute, Federal University of Pará, Brazil; Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Brazil.
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Brazil.
Cryobiology. 2018 Aug;83:97-99. doi: 10.1016/j.cryobiol.2018.06.006. Epub 2018 Jun 13.
We aimed to evaluate the effect of three extracellular cryoprotectants on the morphology of vitrified feline preantral follicles. Feline ovarian fragments (0.5 × 2 × 2 mm) collected from five domestic adult cats subjected to ovariohysterectomy for routine castration were vitrified with ethylene glycol (EG) 40% combined or not with sucrose (0.1 or 0.5 M), trehalose (0.1 or 0.5 M), or raffinose (0.1 M). After vitrification using the solid-surface method and warming of the tissues, cryoprotectants were washed out of the ovarian tissues, which were fixed for histological analysis. The percentages of normal follicles were similar to the control (fresh) (62.9 ± 4.1%) only for tissues exposed and cryopreserved with EG + trehalose at concentrations of 0.1 (35.8 ± 8.3%) and 0.5 M (33.4 ± 5.4%). All the other sugars decreased the percentages of morphologically normal follicles as compared to the control group and the trehalose groups. Based on the results of the present study, we recommend the use of trehalose as the extracellular cryoprotectant for the vitrification of feline ovarian tissue.
我们旨在评估三种细胞外冷冻保护剂对玻璃化猫窦前卵泡形态的影响。从五只因常规去势而接受卵巢子宫切除术的成年家猫收集的猫卵巢组织块（0.5×2×2毫米），用40%乙二醇（EG）联合或不联合蔗糖（0.1或0.5M）、海藻糖（0.1或0.5M）或棉子糖（0.1M）进行玻璃化处理。使用固体表面法进行玻璃化处理并使组织复温后，将冷冻保护剂从卵巢组织中洗去，然后将组织固定用于组织学分析。仅对于用0.1（35.8±8.3%）和0.5M（33.4±5.4%）浓度的EG+海藻糖处理并冷冻保存的组织，正常卵泡的百分比与对照组（新鲜组织）相似（62.9±4.1%）。与对照组和海藻糖组相比，所有其他糖类均降低了形态正常卵泡的百分比。基于本研究结果，我们推荐使用海藻糖作为猫卵巢组织玻璃化的细胞外冷冻保护剂。
