Watanabe Y, Amino N, Tamaki H, Iwatani Y, Miyai K
Endocrinol Jpn. 1985 Feb;32(1):81-8. doi: 10.1507/endocrj1954.32.81.
The effects of bovine thyrotropin (TSH) on DNA synthesis and cyclic AMP production were studied in porcine thyroid follicles using suspension culture. During the early 72 hours incubation, the time-dependent uptake of [3H]thymidine by the follicles was observed. In the presence of 10 mU/ml TSH, the uptake of [3H] thymidine was significantly depressed at 72 hours incubation. TSH inhibition of [3H] thymidine incorporation was related to its concentration and the 50% inhibition was observed by using 1.0 mU/ml TSH. Under the same conditions, cyclic AMP production was stimulated by TSH and the stimulation was observed to be related to TSH concentration. In these experiments, the incubation time was 30 min. Dibutyryl cyclic AMP, an analogue of cyclic AMP, inhibited the [3H] thymidine uptake at 72 hours incubation. From these results, it is suggested that TSH inhibits DNA synthesis, and that the inhibition may be mediated by cyclic AMP that is produced by TSH stimulation.
采用悬浮培养法，研究了牛促甲状腺激素（TSH）对猪甲状腺滤泡中DNA合成及环磷酸腺苷（cAMP）生成的影响。在培养的最初72小时内，观察到滤泡对[3H]胸腺嘧啶核苷的时间依赖性摄取。在10 mU/ml TSH存在的情况下，培养72小时时，[3H]胸腺嘧啶核苷的摄取显著降低。TSH对[3H]胸腺嘧啶核苷掺入的抑制作用与其浓度有关，使用1.0 mU/ml TSH时观察到50%的抑制率。在相同条件下，TSH刺激了cAMP的生成，且观察到这种刺激与TSH浓度有关。在这些实验中，孵育时间为30分钟。二丁酰环磷酸腺苷（一种环磷酸腺苷类似物）在孵育72小时时抑制了[3H]胸腺嘧啶核苷的摄取。从这些结果表明，TSH抑制DNA合成，且这种抑制可能由TSH刺激产生的环磷酸腺苷介导。
