Saidoune-Malek Imane, Ait-Lounis Aouatef, Laraba-Djebari Fatima
USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria.
USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria.
Acta Trop. 2018 Sep;185:307-313. doi: 10.1016/j.actatropica.2018.06.013. Epub 2018 Jun 21.
We previously reported that Androctonus australis hector (Aah) venom induces inflammation in several tissues, however limited information is available on its role in gastrointestinal tract. Here we evaluate the involvement of TNF-α in lipid metabolism in the small intestine after Aah envenomation. To address these issues, NMRI mice (3-month-old) were pre-treated with a TNF-α antagonist, 30 min prior to Aah venom injection. Redox status, cytotoxicity and histopathological changes were analyzed in small intestine 3 and 24 h after Aah injection. Lipid metabolism was evaluated through lipid tolerance test in sera. Lipid content in small intestine was also evaluated at different times after envenomation. Obtained results showed that Aah venom affects the intestinal integrity. This cytotoxicity could be associated with lipid peroxidation and altered or insufficient antioxidant system. These results also highlight the perturbation of lipid absorption in intestine tissue of envenomed mice. The use of TNF-α antagonist prior to Aah venom injection seems to be able to improve lipid profile, oxidative stress and antioxidant activity. These findings suggest that Aah venom induces lipid alterations in the intestinal tissue mechanisms involving of TNF- α.
我们之前报道过，澳毒蛛（Androctonus australis hector，Aah）毒液会在多个组织中引发炎症，然而关于其在胃肠道中的作用，现有信息有限。在此，我们评估肿瘤坏死因子-α（TNF-α）在Aah毒液注入后小肠脂质代谢中的作用。为解决这些问题，在注射Aah毒液前30分钟，用TNF-α拮抗剂对NMRI小鼠（3月龄）进行预处理。在Aah注射后3小时和24小时，分析小肠中的氧化还原状态、细胞毒性和组织病理学变化。通过血清脂质耐量试验评估脂质代谢。还在毒液注入后的不同时间评估小肠中的脂质含量。获得的结果表明，Aah毒液会影响肠道完整性。这种细胞毒性可能与脂质过氧化以及抗氧化系统改变或不足有关。这些结果还突出了被毒液注入小鼠的肠道组织中脂质吸收的紊乱。在注射Aah毒液前使用TNF-α拮抗剂似乎能够改善脂质状况、氧化应激和抗氧化活性。这些发现表明，Aah毒液通过涉及TNF-α的机制在肠道组织中诱导脂质改变。
