Satué K, Marcilla M, Medica P, Cravana C, Fazio E
Department of Animal Medicine and Surgery, Faculty of Veterinary, Cardenal Herrera-CEU University, Valencia, Spain.
Department of Animal Medicine and Surgery, Faculty of Veterinary, Cardenal Herrera-CEU University, Valencia, Spain.
Theriogenology. 2018 Sep 15;118:164-171. doi: 10.1016/j.theriogenology.2018.05.040. Epub 2018 May 30.
Growth hormone (GH) and insulin-like growth factor (IGF-I) exert essential functions to maintain an adequate metabolic activity for correct placental and fetal development. Although fructosamine (FRUCT) is used in clinical evaluation to assess the glycaemic state, the relationships between GH, IGF-I and FRUCT remain unknown in the mare. Therefore, the objectives of this study were to evaluate the relationships among these parameters in pregnant mares. A total of 30 healthy Spanish Purebred mares, with an age range of 9.33 ± 3.31 years, were studied during the 11 months of gestation. Serum concentrations of GH, IGF-I and FRUCT were analyzed by EIA, competition ELISA and spectrophotometry, respectively. GH showed a biphasic pattern, the first occurred during 4th and 5th month and the second which was higher than the 1st one during the 7th, 8th and 9th month (P < 0.05). Compared to the 1st month, the IGF-I concentrations increased in the 2nd month (P < 0.05), decreased in the 3rd and 4th month and increased to reach the maximum average value in the 5th month (P < 0.05); after slight oscillations between the 6th and 8th month, IGF-I concentrations increased in the 9th month (P < 0.05), decreasing until the end of gestation. FRUCT increased progressively and significantly from the 6th month of gestation, reaching the maximum values in the last month of gestation (P < 0.05). These results suggest that alternative mechanisms other than GH and IGF-I could be involved in the regulation of glycaemic metabolism in pregnant mare.
生长激素（GH）和胰岛素样生长因子（IGF-I）发挥着重要作用，以维持适当的代谢活动，促进胎盘和胎儿的正常发育。虽然果糖胺（FRUCT）用于临床评估以评估血糖状态，但在母马中，GH、IGF-I和FRUCT之间的关系尚不清楚。因此，本研究的目的是评估妊娠母马中这些参数之间的关系。在妊娠的11个月期间，对30匹健康的西班牙纯种母马进行了研究，年龄范围为9.33±3.31岁。分别通过酶免疫分析（EIA）、竞争酶联免疫吸附测定（ELISA）和分光光度法分析血清中GH、IGF-I和FRUCT的浓度。GH呈现双相模式，第一次出现在第4和第5个月，第二次出现在第7、第8和第9个月，且高于第一次（P<0.05）。与第1个月相比，IGF-I浓度在第2个月增加（P<0.05），在第3和第4个月降低，并在第5个月增加至达到最大平均值（P<0.05）；在第6和第8个月之间略有波动后，IGF-I浓度在第9个月增加（P<0.05），直至妊娠结束时降低。FRUCT从妊娠第6个月开始逐渐显著增加，在妊娠最后一个月达到最大值（P<0.05）。这些结果表明，除了GH和IGF-I之外，其他替代机制可能参与妊娠母马血糖代谢的调节。
