Subramaniam Akila, Van Der Pol William J, Ptacek Travis, Lobashevsky Elena, Neely Cherry, Biggio Joseph R, Lefkowitz Elliot J, Morrow Casey D, Edwards Rodney K
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Center for Women's Reproductive Health, University of Alabama at Birmingham, Birmingham, AL, USA.
Biomedical Informatics, Center for Clinical and Translational Sciences, University of Oklahoma College of Medicine, Oklahoma City, OK, USA.
J Matern Fetal Neonatal Med. 2020 Feb;33(3):359-367. doi: 10.1080/14767058.2018.1490721. Epub 2018 Jul 22.
: To evaluate if midtrimester maternal serum contains microbial DNA and whether it differs between women with spontaneous preterm birth (SPTB) and those delivering at term.: In this retrospective case-control study, we identified 20 healthy nulliparas with SPTB at 24-33 weeks of a nonanomalous singleton in 2014. Each case was matched by race/ethnicity to a control delivering at 39-40 weeks. Serum samples, collected at 15-20 weeks and stored at -80 C, were thawed and DNA extracted. PCR with primers targeting the 16S rDNA V4 region were used to prepare an amplicon library, sequenced using Illumina MiSeq, and analyzed using quantitative insight into microbial ecology (QIIME). Taxonomy was assigned using Ribosomal Database program (RDP) Classifier (threshold 0.8) against a modified Greengenes database. Differences in number of observed species, microbial alpha-diversity and beta-diversity, and taxa level analyses were undertaken. All 40 samples were included. Women with SPTB had more unique observed species ( = .046) and higher mean alpha-diversity by Shannon index (but not Chao1 or Simpson) ( = .024). Microbial composition was different between groups by Bray-Curtis clustering ( = .03) but not by weighted ( = .13) or unweighted Unifrac ( = .11). Numerous taxa in the Firmicutes, Proteobacteria, and Actinobacteria phyla differed between groups ( < .05). SPTB is associated with distinct microbial DNA changes detected in midtrimester maternal serum.
评估孕中期孕妇血清中是否含有微生物DNA，以及自然早产（SPTB）孕妇与足月分娩孕妇的微生物DNA是否存在差异。：在这项回顾性病例对照研究中，我们确定了20名2014年在24 - 33周时自然早产的健康初产妇，她们均为单胎且无异常。每个病例按照种族/民族与一名在39 - 40周分娩的对照进行匹配。收集在15 - 20周时采集并储存在 - 80°C的血清样本，解冻后提取DNA。使用靶向16S rDNA V4区域的引物进行PCR以制备扩增子文库，使用Illumina MiSeq进行测序，并使用微生物生态学定量洞察（QIIME）进行分析。使用核糖体数据库程序（RDP）分类器（阈值0.8）针对改良的Greengenes数据库进行分类。进行了观察到的物种数量、微生物α多样性和β多样性以及分类水平分析的差异研究。纳入了所有40个样本。自然早产的女性有更多独特的观察到的物种（P = 0.046），并且通过香农指数（但不是Chao1或辛普森指数）计算的平均α多样性更高（P = 0.024）。通过布雷 - 柯蒂斯聚类分析，两组之间的微生物组成不同（P = 0.03），但加权（P = 0.13）或非加权的UniFrac分析（P = 0.11）显示无差异。厚壁菌门、变形菌门和放线菌门中的许多分类群在两组之间存在差异（P < 0.05）。自然早产与孕中期孕妇血清中检测到的不同微生物DNA变化有关。
