Inoue Akira
Hokkaido University, Hakodate, Japan.
Methods Enzymol. 2018;605:499-524. doi: 10.1016/bs.mie.2018.01.030. Epub 2018 Mar 12.
Alginate, an anionic heteropolysaccharide extracted from natural brown algae, has useful properties for the food, chemical, medical, and agricultural industries. Degradation of alginate by alginate lyase is a key process to produce unsaturated oligoalginate and unsaturated monosaccharide 4-deoxy-l-erythro-5-hexoseulose uronic acid. Alginate lyases belonging to the polysaccharide lyase family 7 have been found in, and isolated from, organisms thriving in various environments. Furthermore, research on their function and structure has also progressed well. Here, the preparation of native and recombinant PL-7 alginate lyases and the methods for evaluation of enzymatic activity are summarized. Examples of PL-7 alginate lyase applications are also described.
海藻酸盐是一种从天然褐藻中提取的阴离子杂多糖，对食品、化工、医药和农业等行业具有有用的特性。海藻酸裂解酶催化海藻酸盐降解是生产不饱和低聚海藻酸盐和不饱和单糖4-脱氧-L-赤藓糖-5-己糖醛酸的关键过程。属于多糖裂解酶家族7的海藻酸裂解酶已在各种环境中生长的生物中被发现并分离出来。此外，对其功能和结构的研究也取得了良好进展。在此，总结了天然和重组PL-7海藻酸裂解酶的制备方法以及酶活性评估方法。还介绍了PL-7海藻酸裂解酶的应用实例。
