Moore Angelique N, Haun Cody T, Kephart Wesley C, Holland Angelia M, Mobley Christopher B, Pascoe David D, Roberts Michael D, Martin Jeffrey S
Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine-Auburn Campus, Auburn, AL 36832, USA.
School of Kinesiology, Auburn University, Auburn, AL 36849, USA.
Sports (Basel). 2017 Oct 16;5(4):80. doi: 10.3390/sports5040080.
We examined the acute effect of a red spinach extract (RSE) (1000 mg dose; ~90 mg nitrate (NO 3 - )) on performance markers during graded exercise testing (GXT). For this randomized, double-blind, placebo (PBO)-controlled, crossover study, 15 recreationally-active participants (aged 23.1 ± 3.3 years; BMI: 27.2 ± 3.7 kg/m²) reported >2 h post-prandial and performed GXT 65⁻75 min post-RSE or PBO ingestion. Blood samples were collected at baseline (BL), pre-GXT (65⁻75 min post-ingestion; PRE), and immediately post-GXT (POST). GXT commenced with continuous analysis of expired gases. Plasma concentrations of NO 3 - increased PRE (+447 ± 294%; < 0.001) and POST (+378 ± 179%; < 0.001) GXT with RSE, but not with PBO (+3 ± 26%, -8 ± 24%, respectively; > 0.05). No effect on circulating nitrite (NO 2 - ) was observed with RSE (+3.3 ± 7.5%, +7.7 ± 11.8% PRE and POST, respectively; > 0.05) or PBO (-0.5 ± 7.9%, -0.2 ± 8.1% PRE and POST, respectively; > 0.05). When compared to PBO, there was a moderate effect of RSE on plasma NO 2 - at PRE (g = 0.50 [-0.26, 1.24] and POST g = 0.71 [-0.05, 1.48]). During GXT, VO₂ at the ventilatory threshold was significantly higher with RSE compared to PBO (+6.1 ± 7.3%; < 0.05), though time-to-exhaustion (-4.0 ± 7.7%; > 0.05) and maximal aerobic power (i.e., VO₂ peak; -0.8 ± 5.6%; > 0.05) were non-significantly lower with RSE. RSE as a nutritional supplement may elicit an ergogenic response by delaying the ventilatory threshold.
我们研究了红菠菜提取物（RSE）（1000毫克剂量；约90毫克硝酸盐（NO₃⁻））在分级运动测试（GXT）期间对运动表现指标的急性影响。在这项随机、双盲、安慰剂（PBO）对照的交叉研究中，15名有休闲运动习惯的参与者（年龄23.1±3.3岁；体重指数：27.2±3.7千克/平方米）在餐后2小时以上报告情况，并在摄入RSE或PBO后65⁻75分钟进行GXT。在基线（BL）、GXT前（摄入后65⁻75分钟；PRE）和GXT后立即（POST）采集血样。GXT开始时持续分析呼出气体。RSE组在GXT前（+447±294%；P<0.001）和GXT后（+378±179%；P<0.001）血浆NO₃⁻浓度升高，而PBO组分别为+3±26%、-8±24%（P>0.05），无升高。RSE组（分别在PRE和POST时为+3.3±（续）7.5%、+7.7±11.8%；P>0.05）和PBO组（分别在PRE和POST时为-0.5±7.9%、-0.2±8.1%；P>0.05）对循环亚硝酸盐（NO₂⁻）均无影响。与PBO相比，RSE在PRE时对血浆NO₂⁻有中等程度影响（g=0.50[-0.26,1.24]），在POST时g=0.71[-0.05,1.48]）。在GXT期间，与PBO相比，RSE组在通气阈值时的VO₂显著更高（+6.1±7.3%；P<0.05），尽管RSE组的运动至疲劳时间（-4.0±7.7%；P>0.05）和最大有氧功率（即VO₂峰值；-0.8±5.6%；P>0.05）无显著降低。RSE作为一种营养补充剂可能通过延迟通气阈值引发促力反应。
