Sansonno D E, Minoia G M, Detomaso P, Manghisi O G
Hepatogastroenterology. 1985 Jun;32(3):121-5.
Binding activity for polymerized human serum albumin (pHSA-binding), studied in passive haemoagglutination, receptors for polymerized human serum albumin (pHSA-receptors), studied in ELISA, as well as the circulating IgM/HBs complex were tested in 71 chronic carriers of HBsAg with and without liver pathology. We found that 73.2% of the sera were reactive for pHSA-binding while 45% were reactive for pHSA-receptors and 42.2% for the circulating IgM/HBs complex. The distribution and mode of association of these 3 markers showed a close correlation with the e-antigen in circulation (HBeAg) and with liver disease (p less than 0.05). We further observed that pHSA-binding can be present in the absence of pHSA-receptors, suggesting the possible existence of further reactants in the serum-pHSA reaction. We did not observe any correlations between the circulating IgM/HBs complexes, pHSA-binding and pHSA-receptors. Blocking experiments, in fact, confirmed the non-involvement of polymerized human serum albumin in the formation of the circulating IgM/HBs complex. Elution experiments showed that, in addition to HBsAg, class-G immunoglobulins are also directly involved in the serum-pHSA reaction.
采用被动血凝试验研究聚合人血清白蛋白结合活性（pHSA结合），采用酶联免疫吸附测定法研究聚合人血清白蛋白受体（pHSA受体），并对71例有或无肝脏病变的HBsAg慢性携带者检测循环IgM/HBs复合物。我们发现，73.2%的血清对pHSA结合呈反应性，45%对pHSA受体呈反应性，42.2%对循环IgM/HBs复合物呈反应性。这三种标志物的分布和关联模式与循环中的e抗原（HBeAg）和肝脏疾病密切相关（P<0.05）。我们进一步观察到，在没有pHSA受体的情况下也可出现pHSA结合，提示血清-pHSA反应中可能存在其他反应物。我们未观察到循环IgM/HBs复合物、pHSA结合与pHSA受体之间存在任何相关性。事实上，阻断实验证实聚合人血清白蛋白不参与循环IgM/HBs复合物的形成。洗脱实验表明，除HBsAg外，G类免疫球蛋白也直接参与血清-pHSA反应。
