Das Tanmoy, Banerjee Amrita
1School of Crop Protection, College of Post Graduate Studies, Central Agricultural University, Umiam, Meghalaya 793103 India.
2ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya 793103 India.
Virusdisease. 2018 Jun;29(2):157-166. doi: 10.1007/s13337-018-0451-7. Epub 2018 May 2.
Banana bunchy top virus (BBTV) is a serious threat to banana ( spp.) production in India. Generally, BBTV isolates within the country share very low genetic diversity. However, in India, relatively greater diversity has been observed between isolates from north-eastern (NE) region (Meghalaya) and rest of India. Tripura is situated in the south-west corner of NE India and shares international border with Bangladesh. During 2014-2015, diagnostic surveys were conducted in seven districts of Tripura and polymerase chain reaction based detection established that BBTD is widely prevalent in all parts of Tripura showing an average incidence of 22.02%. Among the cultivars, maximum BBTV infection (27.03%) was recorded in 'Chini Champa', followed by plantain (24.29%). A representative population (31 isolates) of BBTV from Tripura was characterized based on DNA R and DNA S. Phylogenetic analysis based on BBTV DNA R and DNA S generated two broad clusters of Pacific-Indian Oceans (PIO) and south-east Asian groups including all Tripura isolates within PIO cluster. The clustering pattern and genetic diversity of BBTV population from Tripura suggested monophyletic origin of majority of representative isolates from a common ancestor of PIO group. The exchange of vegetative propagules within and in between countries could have contributed to the geographical expansion of PIO isolates in Tripura. However, four variant BBTV isolates has been identified from North Tripura and Khowai districts possessing somewhat unique variability than that of distinct isolate (BBTV-Umiam) reported from NE India (Meghalaya).
香蕉束顶病毒（BBTV）对印度的香蕉（品种）生产构成严重威胁。一般来说，该国境内的BBTV分离株遗传多样性很低。然而，在印度，东北部（NE）地区（梅加拉亚邦）与印度其他地区的分离株之间观察到了相对较大的多样性。特里普拉邦位于印度东北部的西南角，与孟加拉国接壤。在2014 - 2015年期间，对特里普拉邦的七个区进行了诊断调查，基于聚合酶链反应的检测确定BBTD在特里普拉邦各地广泛流行，平均发病率为22.02%。在这些品种中，“Chini Champa”的BBTV感染率最高（27.03%），其次是大蕉（24.29%）。基于DNA R和DNA S对来自特里普拉邦的一组代表性BBTV群体（31个分离株）进行了特征分析。基于BBTV DNA R和DNA S的系统发育分析产生了太平洋 - 印度洋（PIO）和东南亚组的两个广泛聚类，所有特里普拉邦分离株都在PIO聚类中。来自特里普拉邦的BBTV群体的聚类模式和遗传多样性表明，大多数代表性分离株起源于PIO组的一个共同祖先的单系起源。国家内部和国家之间营养繁殖体的交换可能促成了PIO分离株在特里普拉邦的地理扩张。然而，已从北特里普拉邦和科瓦伊区鉴定出四个变异的BBTV分离株，它们具有比印度东北部（梅加拉亚邦）报道的独特分离株（BBTV - 乌米亚姆） somewhat unique variability 。 （注：原文中“somewhat unique variability”表述不太准确，可能是“某种独特的变异性”之类的意思，但按要求未做修改直接翻译）
