miR-22-3p 通过靶向烯醇化酶 1 调节视网膜母细胞瘤细胞的增殖。
MiR-22-3p targeting alpha-enolase 1 regulates the proliferation of retinoblastoma cells.
机构信息
Department of Ophthalmology, Third Affiliated Hospital of Soochow University, China.
Department of Ophthalmology, Third Affiliated Hospital of Soochow University, China.
出版信息
Biomed Pharmacother. 2018 Sep;105:805-812. doi: 10.1016/j.biopha.2018.06.038. Epub 2018 Jun 15.
OBJECTIVE
This study sought to explore the role of alpha-Enolase 1 (ENO1) in retinoblastoma (RB) which remains uncertain.
METHODS
The expression of ENO1 in RB cell lines was examined by RT-qPCR and western blot. The biological function of ENO1 on cell proliferation in RB was determined in vitro. The predicted target of ENO1 was validated by dual-luciferase reporter assay and rescue experiment. The validation of clinical tissue samples was performed by RT-qPCR.
RESULTS
Elevated ENO1 could promote the proliferation of RB cells. The dual luciferase reporter assay confirmed that ENO1 is the target for miR-22-3p. In rescue experiment, the result also indicated that miR-22-3p inhibits the proliferation of RB cells by negatively regulating the expression of ENO1. These differences were statistically significant (P<0.05).
CONCLUSION
ENO1 functions as an oncogene in RB and inhibiting ENO1 by miR-22-3p suppresses the proliferation of RB cell lines. miR-22-3p/ENO1 pathway may serve as a novel target in RB.
目的
本研究旨在探索烯醇化酶 1（ENO1）在视网膜母细胞瘤（RB）中的作用，但其作用尚不确定。
方法
通过 RT-qPCR 和 Western blot 检测 RB 细胞系中 ENO1 的表达。通过体外实验确定 ENO1 对 RB 细胞增殖的生物学功能。通过双荧光素酶报告基因检测和挽救实验验证 ENO1 的预测靶标。通过 RT-qPCR 验证临床组织样本。
结果
升高的 ENO1 可促进 RB 细胞的增殖。双荧光素酶报告基因检测证实 ENO1 是 miR-22-3p 的靶基因。在挽救实验中，结果也表明 miR-22-3p 通过负调控 ENO1 的表达抑制 RB 细胞的增殖。这些差异具有统计学意义（P<0.05）。
结论
ENO1 在 RB 中作为癌基因发挥作用，miR-22-3p 通过抑制 ENO1 抑制 RB 细胞系的增殖。miR-22-3p/ENO1 通路可能成为 RB 的一个新靶点。
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