Liao Kai, Sun Lingmei
Department of Pathology and Pathophysiology, Medical School of Southeast University, Nanjing 210009, P.R. China.
Department of Pharmacology, Medical School of Southeast University, Nanjing 210009, P.R. China.
J Microbiol Biotechnol. 2018 Jul 28;28(7):1086-1093. doi: 10.4014/jmb.1801.01024.
Honokiol, a bioactive compound isolated from the cone and bark of , has been shown to have various activities including inhibition of the growth of . We investigated the roles of the Hsp90-calcineurin pathway in the antifungal activity of honokiol. The pharmacologic tool was employed to evaluate the effects of Hsp90 and calcineurin in the antifungal activity of honokiol. We also evaluated the protective effects of the calcineurin inhibitor cyclosporin A (CsA) on honokiol-induced mitochondrial dysfunction by the fluorescence staining method. The Hsp90 inhibitor potentiated the antifungal activity of honokiol. A strain with the calcineurin gene deleted displayed enhanced sensitivity to honokiol. However, co-treatment with calcineurin inhibitor CsA attenuated the cytotoxic activity of honokiol due to the protective effect on mitochondria. Our results provide insight into the action mechanism of honokiol.
厚朴酚是一种从[具体植物]的球果和树皮中分离出的生物活性化合物，已被证明具有多种活性，包括抑制[具体对象]的生长。我们研究了热休克蛋白90（Hsp90）-钙调神经磷酸酶途径在厚朴酚抗真菌活性中的作用。使用药理学工具评估Hsp90和钙调神经磷酸酶在厚朴酚抗真菌活性中的作用。我们还通过荧光染色法评估了钙调神经磷酸酶抑制剂环孢素A（CsA）对厚朴酚诱导的线粒体功能障碍的保护作用。Hsp90抑制剂增强了厚朴酚的抗真菌活性。一株缺失钙调神经磷酸酶基因的菌株对厚朴酚表现出更高的敏感性。然而，由于对线粒体的保护作用，钙调神经磷酸酶抑制剂CsA与厚朴酚联合处理减弱了厚朴酚的细胞毒性活性。我们的结果为厚朴酚的作用机制提供了见解。
