Inoko H, Ando A, Kimura M, Tsuji K
J Immunol. 1985 Sep;135(3):2156-9.
From a human cDNA library constructed from a consanguineous HLA-homozygous cell line, AKIBA (HLA-A24, Bw52, DR2, Dw12, DQw1, and Cp63) (Cp63, a new SB type), a cDNA clone encoding a new HLA class II antigen heavy chain named DQ alpha was isolated, and was analyzed by Southern blot hybridization and by nucleotide sequence determination. The nucleotide sequence of the DO alpha cDNA was distinct from those of the DR alpha, the DQ alpha, and the DP alpha cDNA, but showed some characteristic features of the class II antigen alpha-chains. We also isolated and identified genomic clones specifying the DO alpha gene. Genomic analyses of cell lines with different HLA-DR serotypes with the use of the DO alpha cDNA as a probe indicated the existence of a single DO alpha gene that exhibited little restriction enzyme polymorphism.
从由近亲HLA纯合细胞系AKIBA（HLA - A24、Bw52、DR2、Dw12、DQw1和Cp63）（Cp63，一种新的SB类型）构建的人cDNA文库中，分离出一个编码名为DQα的新HLA II类抗原重链的cDNA克隆，并通过Southern印迹杂交和核苷酸序列测定进行分析。DQα cDNA的核苷酸序列与DRα、DQα和DPα cDNA的序列不同，但显示出II类抗原α链的一些特征。我们还分离并鉴定了指定DQα基因的基因组克隆。使用DQα cDNA作为探针，对具有不同HLA - DR血清型的细胞系进行基因组分析，结果表明存在单个DQα基因，该基因表现出很少的限制性酶多态性。
