副球菌细胞色素c氧化酶亚基II的克隆
Cloning of Paracoccus cytochrome c oxidase subunit II.
作者信息
Paetow B, Panskus G, Ludwig B
出版信息
J Inorg Biochem. 1985 Mar-Apr;23(3-4):183-6. doi: 10.1016/0162-0134(85)85024-8.
PMID:2991456
Abstract
Cytochrome c oxidase from Paracoccus denitrificans is composed of two subunits, yet is active in both electron transport and proton translocation. A cloning approach and immunologic screening protocol is described for the isolation of the subunit II gene expressed in E. coli. DNA sequencing should establish the extent of homology to eukaryotic oxidase.
摘要
反硝化副球菌的细胞色素c氧化酶由两个亚基组成，但在电子传递和质子转运中均具有活性。本文描述了一种用于分离在大肠杆菌中表达的亚基II基因的克隆方法和免疫筛选方案。DNA测序应能确定与真核生物氧化酶的同源程度。
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