Wrigglesworth J M
J Inorg Biochem. 1985 Mar-Apr;23(3-4):311-6. doi: 10.1016/0162-0134(85)85040-6.
Proteoliposomes incorporating cytochrome c oxidase have been prepared by the cholate dialysis method and by sonication. Sonication produces multilamellar vesicles heterogeneous in size in contrast to a more uniform preparation of unilamellar vesicles produced by the dialysis procedure. Respiratory control in both preparations ranges between 4 and 8. From an electron microscopic analysis of proteoliposome size, the average electrical capacitance/vesicle for the dialyzed and sonicated preparations is calculated as 15 X 10(-18) F and 130 X 10(-18) F, respectively. These capacitance values would lead to a quantization of membrane potential generation by the enzyme at 77 mV/turnover for the dialyzed preparation and 9 mV/turnover for sonicated vesicles. It is argued that these differences can explain the dependence of H+ translocation on the number of turnovers of cytochrome c oxidase in dialyzed preparations in contrast to the lack of dependence on number of turnovers in sonicated preparations.
已通过胆酸盐透析法和超声处理法制备了包含细胞色素c氧化酶的蛋白脂质体。与透析法制备的更均匀的单层囊泡相比，超声处理产生的多层囊泡大小不均一。两种制剂中的呼吸控制范围在4至8之间。通过对蛋白脂质体大小的电子显微镜分析，透析制剂和超声处理制剂的平均电容/囊泡分别计算为15×10⁻¹⁸F和130×10⁻¹⁸F。这些电容值将导致透析制剂中酶产生膜电位的量子化为每周转77 mV，超声处理囊泡为每周转9 mV。有人认为，这些差异可以解释与超声处理制剂中对周转数缺乏依赖性相反，H⁺转运对透析制剂中细胞色素c氧化酶周转数的依赖性。
