Takei Takayuki, Sakoguchi Shogo, Yoshida Masahiro
Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
J Biosci Bioeng. 2018 Nov;126(5):649-652. doi: 10.1016/j.jbiosc.2018.05.020. Epub 2018 Jun 18.
Magnetic manipulation of paramagnetic particles had great potential for efficient bioprocessing. In this study, we stirred microliter-volume water droplets formed on a superhydrophobic surface as micro-bioreactors by using paramagnetic magnetite microparticles manipulated by an external magnetic field. We showed that magnetite microparticles in the droplets spontaneously formed rod-like aggregates, which were like commercial stir bars, in an external magnetic field and spun with rotating of magnetic field. Increasing the rotating rate of the magnetic field and increasing the concentration of the microparticles caused the microparticles to fixate at the air/water interface of the droplets and their rotation at the interface with rotating of magnetic field. The active mixing enhanced the enzyme reaction and microorganism proliferation in the droplets. These results demonstrated that manipulating the magnetite microparticles by an external magnetic field efficiently mixed the small droplets as micro-bioreactors.
顺磁性颗粒的磁性操控在高效生物处理方面具有巨大潜力。在本研究中，我们通过使用外部磁场操控的顺磁性磁铁矿微粒，搅拌在超疏水表面形成的微升体积水滴作为微型生物反应器。我们发现，液滴中的磁铁矿微粒在外部磁场中自发形成棒状聚集体，类似于商业搅拌棒，并随着磁场旋转而旋转。提高磁场的旋转速率和增加微粒浓度会使微粒固定在液滴的空气/水界面处，并随着磁场旋转在界面处旋转。这种主动混合增强了液滴中的酶反应和微生物增殖。这些结果表明，通过外部磁场操控磁铁矿微粒可有效地混合作为微型生物反应器的小液滴。
