Shimada Tomoo, Fuji Kentaro, Ichino Takuji, Teh Ooi-Kock, Koumoto Yasuko, Hara-Nishimura Ikuko
Graduate School of Science, Kyoto University, Kyoto, Japan.
Faculty of Science and Engineering, Konan University, Kobe, Japan.
Methods Mol Biol. 2018;1789:1-7. doi: 10.1007/978-1-4939-7856-4_1.
Vacuolar trafficking plays a vital role in plant growth and development. In this chapter, we describe a powerful technique for the evaluation of vacuolar protein trafficking, which is designated as GREEN FLUORESCENT SEED. Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.
液泡运输在植物生长发育中起着至关重要的作用。在本章中，我们描述了一种用于评估液泡蛋白运输的强大技术，即绿色荧光种子法。基于拟南芥种子中靶向液泡的绿色荧光蛋白，该方法能够无损分离液泡运输缺陷的突变种子并对其进行可视化表征。
