Rangiah Kannan, Palakodeti Dasaradhi
Food Safety & Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute (CFTRI), Mysore, 570020, Karnataka, India.
Institute of Stem Cell Biology and Regenerative Medicine, National Centre for Biological Sciences, GKVK, Bangalore, India.
Methods Mol Biol. 2018;1774:555-570. doi: 10.1007/978-1-4939-7802-1_25.
Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of regenerating large portions of missing body parts. It is one of the best model systems available to address the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from planarians is imperative to understand their role in the regeneration processes. Here we describe a stable isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters (NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact and regenerating planarians.
淡水涡虫物种地中海真涡虫（Schmidtea mediterranea）因其能够再生大部分缺失的身体部位，成为一种新兴的干细胞模型。它是研究再生过程中基本生物学机制的最佳模型系统之一。对涡虫代谢物进行绝对定量对于理解它们在再生过程中的作用至关重要。在此，我们描述了一种稳定同位素稀释超高效液相色谱/质谱/选择反应监测（UHPLC-MS/SRM）分析方法，用于灵敏且定量地评估涡虫中的神经递质（NTs）。我们使用该方法同时定量完整涡虫和再生涡虫中的16种神经递质。
