转甲基化抑制剂3-脱氮腺苷可在体外抑制由人绒毛膜促性腺激素刺激的大鼠睾丸间质细胞产生睾酮。

The transmethylations inhibitor 3-deazaadenosine, inhibits in vitro testosterone production by rat testis interstitial cells stimulated with HCG.

作者信息

Llanos M N, Ronco A M, Pino A M, Valladares L E

出版信息

J Steroid Biochem. 1985 Jul;23(1):73-6. doi: 10.1016/0022-4731(85)90262-6.

Abstract

3-Deazaadenosine (3-DZA), an inhibitor of somatic cell transmethylations, inhibited in vitro HCG-stimulated testosterone synthesis by rat testis interstitial cells. A maximal inhibition of 50% was observed with 100 microM 3-DZA; in addition homocysteine-thiolactone (Hcy) enhanced the inhibitory effect of 3-DZA. On the other hand, when cells were stimulated with dibutyryl cyclic AMP (Bt)2-cAMP, 3-DZA did not exert any effect on the stimulation. The presence of 3-DZA in the incubation medium neither modified HCG Kd values nor the number of its binding sites to Leydig cells. These results demonstrate that inhibitors of transmethylation reactions interfere with hormone-stimulated testosterone synthesis, suggesting that those reactions (presumably phospholipid methylation) at the plasma membrane level are involved in hormone-stimulated testosterone synthesis by rat Leydig cells.

摘要

3-脱氮腺苷（3-DZA），一种体细胞转甲基化抑制剂，在体外可抑制大鼠睾丸间质细胞受促性腺激素释放激素（HCG）刺激的睾酮合成。在100微摩尔3-DZA时观察到最大抑制率为50%；此外，同型半胱氨酸硫内酯（Hcy）增强了3-DZA的抑制作用。另一方面，当细胞用二丁酰环磷腺苷（Bt）2-环磷腺苷刺激时，3-DZA对该刺激没有任何影响。孵育培养基中3-DZA的存在既不改变HCG的解离常数（Kd）值，也不改变其与睾丸间质细胞结合位点的数量。这些结果表明，转甲基化反应抑制剂会干扰激素刺激的睾酮合成，提示在质膜水平上的那些反应（可能是磷脂甲基化）参与了大鼠睾丸间质细胞受激素刺激的睾酮合成。