Chaplinski T J, Sloan G J, Niedel J E
Leuk Res. 1985;9(7):897-903. doi: 10.1016/0145-2126(85)90311-x.
Human promyelocytic leukemia cells (HL-60) mature into functional granulocytes in response to in vitro treatment with several classes of chemical agents. Compounds that increase intracellular adenosine 3':5' -cyclic monophosphate induce a modified program of maturation in which the cells demonstrate functional properties characteristic of mature phagocytic cells while remaining morphologically immature. We compared the developmental programs initiated by two well-studied inducers, retinoic acid and dimethyl sulfoxide, with the programs initiated by two inducers known to raise intracellular adenosine 3':5' -cyclic monophosphate; N6, O2-dibutyryl adenosine 3':5' -cyclic monophosphate and the combination of prostaglandin E2 and theophylline. In response to the increase in intracellular adenosine 3':5' -cyclic monophosphate, the cells ceased proliferation, expressed chemotactic receptors and demonstrated stimulated enzyme release within 24 h. Chemotaxis, adherence, NBT reduction and superoxide production appeared by 72 h, although the cells remained unchanged morphologically. A similar developmental program was induced by dimethylsulfoxide, but appearance of the markers was delayed by 48 h. Expression of these markers was delayed and incomplete in response to retinoic acid.
人早幼粒细胞白血病细胞（HL-60）在体外经几类化学试剂处理后可成熟为有功能的粒细胞。能增加细胞内3':5'-环磷酸腺苷的化合物可诱导一种改良的成熟程序，在此过程中细胞表现出成熟吞噬细胞的功能特性，而形态上仍未成熟。我们将两种研究充分的诱导剂（视黄酸和二甲亚砜）引发的发育程序，与两种已知能提高细胞内3':5'-环磷酸腺苷的诱导剂（N6,O2-二丁酰-3':5'-环磷酸腺苷以及前列腺素E2与茶碱的组合）引发的程序进行了比较。随着细胞内3':5'-环磷酸腺苷增加，细胞在24小时内停止增殖，表达趋化受体，并表现出刺激后的酶释放。趋化性、黏附性、硝基蓝四唑还原以及超氧化物生成在72小时时出现，尽管细胞形态未发生变化。二甲亚砜诱导出了类似的发育程序，但标志物的出现延迟了48小时。视黄酸处理后，这些标志物的表达延迟且不完全。
