Tantanate Chaicharoen, Klinbua Cherdsak
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Lab Med. 2018 Oct 11;49(4):362-368. doi: 10.1093/labmed/lmy030.
To investigate the association between high-fluorescence lymphocyte cell (HFLC) and atypical lymphocyte (AL) counts, and to determine the clinical significance of HFLC.
We compared automated HFLC and microscopic AL counts and analyzed the findings. Patient clinical data for each specimen were reviewed.
A total of 320 blood specimens were included. The correlation between HFLC and microscopic AL counts was 0.865 and 0.893 for absolute and percentage counts, respectively. Sensitivity, specificity, and accuracy of HFLC at the cutoff value of 0.1 × 109 per L for detection of AL were 0.8, 0.77, and 0.8, respectively. Studied patients were classified into 4 groups: infection, immunological disorders, malignant neoplasms, and others. Patients with infections had the highest HFLC. Most of those patients (67.7%) had dengue infection.
HFLC counts were well-correlated with AL counts with the acceptable test characteristics. Applying HFLC flagging may alert laboratory staff to be aware of ALs.
研究高荧光淋巴细胞（HFLC）计数与异型淋巴细胞（AL）计数之间的关联，并确定HFLC的临床意义。
我们比较了自动检测的HFLC计数与显微镜下的AL计数，并对结果进行了分析。对每个标本的患者临床数据进行了回顾。
共纳入320份血液标本。HFLC计数与显微镜下AL计数的相关性，绝对值计数为0.865，百分比计数为0.893。HFLC在检测AL时，截断值为0.1×10⁹/L时的灵敏度、特异度和准确度分别为0.8、0.77和0.8。研究患者分为4组：感染、免疫紊乱、恶性肿瘤和其他。感染患者的HFLC最高。这些患者中的大多数（67.7%）患有登革热感染。
HFLC计数与AL计数具有良好的相关性，检测特征可接受。应用HFLC标记可能会提醒实验室工作人员注意AL的存在。
