Mostafa Naglaa A, Assaf Magda, Elhakim Sami, Abdel-Halim Mona R E, El-Nabarawy Eman, Gharib Khaled
Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Pathology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
J Cutan Pathol. 2018 Nov;45(11):807-816. doi: 10.1111/cup.13305. Epub 2018 Jul 13.
The distinction of trichoepithelioma from basal cell carcinoma in small superficial biopsies is important but often challenging. This has inspired many scientists to test the validity of immunohistochemical markers in the differential diagnosis.
To develop an immunohistochemical protocol that helps in differentiation between both trichoepithelioma (TE) and basal cell carcinoma (BCC) with higher sensitivity and specificity.
Using standard immunohistochemical techniques, we examined 10 TEs and 19 BCCs for the expression of CK19, Ki-67, androgen receptors (AR), CD10, and PHLDA1.
Immunoreactivity of AR, Ki-67, and CD10 in tumor cells was significantly higher in BCC than TE with a diagnostic accuracy in BCC of 75.5%, 75.8%, and 79.3% respectively, whereas immunoreactivity of PHLDA1 in tumor cells and stromal CD10 was significantly higher in TE than BCC with a diagnostic accuracy in TE of 100% and 82.8%, respectively. In contrast, immunoreactivity for CK19 showed no statistically significant differences between both tumors.
The analysis of CD10, Ki-67, and PHLDA1 can be used as a helpful immunohistochemical panel in the distinction between TE and BCC especially in small and superficial biopsies.
在小的浅表活检组织中区分毛发上皮瘤和基底细胞癌很重要，但往往具有挑战性。这促使许多科学家测试免疫组化标志物在鉴别诊断中的有效性。
制定一种免疫组化方案，以更高的敏感性和特异性帮助区分毛发上皮瘤（TE）和基底细胞癌（BCC）。
采用标准免疫组化技术，我们检测了10例毛发上皮瘤和19例基底细胞癌中细胞角蛋白19（CK19）、Ki-67、雄激素受体（AR）、CD10和母源表达印记基因1（PHLDA1）的表达情况。
基底细胞癌中肿瘤细胞的AR、Ki-67和CD10免疫反应性显著高于毛发上皮瘤，其在基底细胞癌中的诊断准确率分别为75.5%、75.8%和79.3%，而毛发上皮瘤中肿瘤细胞的PHLDA1免疫反应性和基质CD10显著高于基底细胞癌，其在毛发上皮瘤中的诊断准确率分别为100%和82.8%。相比之下，两种肿瘤中CK19的免疫反应性无统计学差异。
CD10、Ki-67和PHLDA1的分析可作为一种有用的免疫组化组合，用于区分毛发上皮瘤和基底细胞癌，尤其是在小的浅表活检组织中。
