Thaweesapphithak Sermporn, Tantrawatpan Chairat, Kheolamai Pakpoom, Tantikanlayaporn Duangrat, Manochantr Sirkul
J Med Assoc Thai. 2016 Jul;99 Suppl 4:S75-83.
Evidence exists indicating that mesenchymal stem cells (MSCs) are promising candidate for therapeutic applications. One major obstacle for their clinical use is the biosafety of fetal bovine serum (FBS), which is a crucial part of all media currently used for culture of MSCs. Although some recent studies recommended substituting FBS with human serum (HS) for the expansion of MSCs for clinical use, the characteristics and functional capacity of the expanded cells has only been partially explored. In addition, limited experience indicates that HS may replace FBS in some but not all culture systems. Currently, relatively little is known about using HS instead of FBS for isolation and expansion of placenta derived MSCs. Therefore, this study aimed to compare the exploit of HS and FBS as a supplement in terms of their impact on biological characteristics of MSCs.
MSCs derived from placenta were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum or 10% human serum. The morphology, the expression of MSC markers, the differentiation ability and the proliferation characteristics were examined.
The results demonstrated that MSCs cultured in DMEM supplemented with 10% HS had similar characteristics to MSCs cultured in DMEM supplemented with 10% FBS. Interestingly, MSCs cultured in DMEM supplemented with 10% HS had greater expansion potential than that of MSCs cultured in DMEM supplemented with 10% FBS.
The results obtained from this study imply some application in the use of HS instead of FBS for expansion of placenta derived MSCs. The HS-expanded MSCs might be useful and safe for use as a therapeutic tool in regenerative medicine.
有证据表明间充质干细胞（MSCs）是具有治疗应用前景的候选细胞。其临床应用的一个主要障碍是胎牛血清（FBS）的生物安全性，而FBS是目前所有用于培养MSCs的培养基的关键成分。尽管最近一些研究建议用人血清（HS）替代FBS用于临床应用中MSCs的扩增，但对扩增后细胞的特性和功能能力仅进行了部分探索。此外，有限的经验表明，HS可能在某些而非所有培养系统中替代FBS。目前，关于使用HS而非FBS分离和扩增胎盘来源的MSCs的了解相对较少。因此，本研究旨在比较HS和FBS作为补充剂对MSCs生物学特性的影响。
将胎盘来源的MSCs培养于添加10%胎牛血清或10%人血清的杜尔贝科改良伊格尔培养基中。检测细胞形态、MSC标志物的表达、分化能力和增殖特性。
结果表明，在添加10% HS的DMEM中培养的MSCs与在添加10% FBS的DMEM中培养的MSCs具有相似的特性。有趣的是，在添加10% HS的DMEM中培养的MSCs比在添加10% FBS的DMEM中培养的MSCs具有更大的扩增潜力。
本研究结果表明在使用HS替代FBS扩增胎盘来源的MSCs方面具有一定应用价值。经HS扩增的MSCs作为再生医学中的治疗工具可能是有用且安全的。
